| Literature DB >> 1518057 |
E Horjales1, M M Altamirano, M L Calcagno, Z Dauter, K Wilson, R C Garratt, G Oliva.
Abstract
Hexameric glucosamine-6-phosphate deaminase from Escherichia coli has been crystallized isomorphously with both phosphate and ammonium sulphate as precipitants, over a wide pH range (6.0 to 9.0). The crystals belong to space group R32 and the cell parameters in the hexagonal setting are a = b = 125.9 A and c = 223.2 A. A complete native data set was collected to 2.1 A resolution. Self-rotation function studies suggest that the hexamers sit on the 3-fold axis and have point group symmetry 32, with a non-crystallographic dyad relating two monomers linked by an interchain disulfide bridge. A possible packing for the unit cell is proposed.Entities:
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Year: 1992 PMID： 1518057 DOI： 10.1016/0022-2836(92)91068-z
Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469