Zhao Wang1, Huan Wang. 1. Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084.
Abstract
OBJECTIVE: To study the inhibitive effect of Limax extract on H14 and inducement effect of apoptosis and differentiation of H14 cells in vitro. METHODS: MTT method, DNA gel electrophoresis, cell stain and microscope observation. RESULTS: After H14 cell line was induced by Limax extract, the growth of H14 cell was inhibited in vitro and the morpha of the cells changed. CONCLUSION: Limax extract had inhibitive effect on H14 cell, partially by apoptosis, and partially by differentiation.
OBJECTIVE: To study the inhibitive effect of Limax extract on H14 and inducement effect of apoptosis and differentiation of H14 cells in vitro. METHODS:MTT method, DNA gel electrophoresis, cell stain and microscope observation. RESULTS: After H14 cell line was induced by Limax extract, the growth of H14 cell was inhibited in vitro and the morpha of the cells changed. CONCLUSION:Limax extract had inhibitive effect on H14 cell, partially by apoptosis, and partially by differentiation.