Literature DB >> 15178390

Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibro sp. 510.

Zhenqing Zhang1, Guangli Yu, Huashi Guan, Xia Zhao, Yuguo Du, Xiaolu Jiang.   

Abstract

Alginate that was purified from the fermentation solution of marine bacteria Vibro sp. 510 under specific reaction conditions was hydrolyzed by alginate lyase. Seven oligosaccharides, including di-, tri- and tetrasaccharides, were isolated through low-pressure, gel-permeation chromatography (LP-GPC) and semipreparative strong-anion exchange (SAX) fast-protein liquid chromatography (FPLC). The oligosaccharide structures were elucidated based on ESIMS and 2D NMR spectral analysis. The hydrolytic specificity of this alginate lyase to alginate is discussed. Copyright 2004 Elsevier Ltd.

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Year:  2004        PMID: 15178390     DOI: 10.1016/j.carres.2004.03.010

Source DB:  PubMed          Journal:  Carbohydr Res        ISSN: 0008-6215            Impact factor:   2.104


  20 in total

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Journal:  Mar Biotechnol (NY)       Date:  2008-06-14       Impact factor: 3.619

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