| Literature DB >> 15177912 |
W Van Dessel1, L Van Mellaert, N Geukens, E Lammertyn, J Anné.
Abstract
Several molecular techniques require high quality RNA, completely free of DNA. Standard methods to isolate total RNA from Streptomyces spp. are based on the application of a 'Modified Kirby Mix' [Practical Streptomyces Genetics. The John Innes Foundation, Norwich, pp. 613]. Here we present an alternative procedure using Triton X-100 and EDTA for the isolation of total RNA from Streptomyces. Copyright 2004 Elsevier B.V.Entities:
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Year: 2004 PMID： 15177912 DOI： 10.1016/j.mimet.2004.03.015
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363