A method for the determination of low-level organic-bound tritium activities in environmental samples.

The authors discuss some of the difficulties encountered when analyzing organically bound tritium (OBT) and describe a sensitive method for low-level OBT determination in biological samples. The methodology, which combines suitable sample treatment, a combustion apparatus for large-sized samples and low-background liquid scintillation counting, provides exclusively carbon-bound tritium measurement. Two key points of this methodology are described and illustrated. The first one is the stage of removal of the exchangeable organic tritium. The efficiency of this stage, carried out by mixing powdered dry samples with tritium-free water, is evaluated. The second key point is the set of precautions taken at any stage of the treatment to avoid contamination of the samples by ambient atmospheric moisture. The detection limit of the method is about 0.5 Bq kg(-1) of dry material. Low-level applications of this methodology are given with estimation of the OBT/HTO ratios.