Literature DB >> 15175854

Development and validation of a PCR-based marker assay for negative selection of the HMW glutenin allele Glu-B1-1d (Bx-6) in wheat.

G Schwarz1, F G Felsenstein, G Wenzel.   

Abstract

Polymorphisms between the coding sequences of high-molecular-weight (HMW) glutenin x-type genes at the Glu-1 locus were used to amplify Glu-1B x-type-specific PCR fragments. PCR analysis in a wheat cultivar subset carrying different Glu-1B x-type alleles resulted in PCR fragments that differed in size for Glu-B1-1d (B-x6) and non -Glu-B1-1d (B-x6) genotypes. Subsequent sequencing analysis revealed a 15-bp in-frame insertion in the coding regions of all Glu-B1-1d (B-x6) genotypes which allowed the development of a B-x6-specific PCR assay for high-throughput allele sizing by ion-pair reversed-phase high-performance liquid chromatography. The assay was validated in a set of 86 German wheat cultivars, and genotyping data unequivocally verified the presence of HMW glutenin subunits GLU-B1-1D (Bx-6) + GLU-B1-2A (By-8) by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis. These results demonstrate that the PCR assay can be applied for the detection and negative selection of the 'poor breadmaking quality' Glu-B1-1d (B-x6) alleles in wheat breeding programs.

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Year:  2004        PMID： 15175854     DOI： 10.1007/s00122-004-1718-5

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN： 0040-5752            Impact factor:   5.699


  12 in total

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Journal:  Nucleic Acids Res       Date:  1985-12-20       Impact factor: 16.971

4.  DNA restriction-fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits of wheat.

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Authors:  J D Thompson; D G Higgins; T J Gibson
Journal:  Nucleic Acids Res       Date:  1994-11-11       Impact factor: 16.971

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Authors:  O D Anderson; F C Greene; R E Yip; N G Halford; P R Shewry; J M Malpica-Romero
Journal:  Nucleic Acids Res       Date:  1989-01-11       Impact factor: 16.971

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Authors:  P R Shewry; S M Gilbert; A W J Savage; A S Tatham; Y-F Wan; P S Belton; N Wellner; R D'Ovidio; F Békés; N G Halford
Journal:  Theor Appl Genet       Date:  2002-10-31       Impact factor: 5.699

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Journal:  Theor Appl Genet       Date:  1994-08       Impact factor: 5.699
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2.  New DNA markers for high molecular weight glutenin subunits in wheat.

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4.  Comprehensive Identification and Bread-Making Quality Evaluation of Common Wheat Somatic Variation Line AS208 on Glutenin Composition.

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Journal:  PLoS One       Date:  2016-01-14       Impact factor: 3.240

5.  SNP markers for low molecular glutenin subunits (LMW-GSs) at the Glu-A3 and Glu-B3 loci in bread wheat.

Authors:  Susanne Dreisigacker; Yonggui Xiao; Deepmala Sehgal; Carlos Guzman; Zhonghu He; Xianchun Xia; Roberto J Peña
Journal:  PLoS One       Date:  2020-05-12       Impact factor: 3.240
  5 in total

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