Literature DB >> 15173620

Assaying phospholipase A2 activity.

Christina C Leslie1, Michael H Gelb.   

Abstract

Mammalian cells contain many structurally and functionally diverse phospholipases A2 (PLA2) that catalyze the hydrolysis of sn-2 fatty acid from membrane phospholipid. Assays are described for measuring the activity of Group IVA cytosolic PLA2alpha(cPLAalpha) and for secreted PLA2s (sPLA2) that are suitable for purified enzymes and for measuring activity in crude cell lysates and culture medium. The assay for cPLA2alpha involves measuring the calcium-dependent release of radiolabeled sn-2 arachidonic acid from small unilamellar vesicles of phosphatidylcholine. Methods are described for distinguishing cPLA2alpha activity in cell lysates from other PLA2s. sPLA2 activity is monitored using a fluorimetric assay that measures the continuous calcium-dependent formation of albumin-bound pyrene fatty acid from the sn-2 position of phosphatidylglycerol.

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Year:  2004        PMID： 15173620     DOI： 10.1385/1-59259-816-1:229

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

1.  Function, activity, and membrane targeting of cytosolic phospholipase A(2)zeta in mouse lung fibroblasts.

Authors:  Moumita Ghosh; Robyn Loper; Farideh Ghomashchi; Dawn E Tucker; Joseph V Bonventre; Michael H Gelb; Christina C Leslie
Journal:  J Biol Chem       Date:  2007-02-11       Impact factor: 5.157

2.  Inherited human cPLA(2alpha) deficiency is associated with impaired eicosanoid biosynthesis, small intestinal ulceration, and platelet dysfunction.

Authors:  David H Adler; Joy D Cogan; John A Phillips; Nathalie Schnetz-Boutaud; Ginger L Milne; Tina Iverson; Jeffrey A Stein; David A Brenner; Jason D Morrow; Olivier Boutaud; John A Oates
Journal:  J Clin Invest       Date:  2008-06       Impact factor: 14.808

3.  The enteropathy of prostaglandin deficiency.

Authors:  David H Adler; John A Phillips; Joy D Cogan; Tina M Iverson; Nathalie Schnetz-Boutaud; Jeffrey A Stein; David A Brenner; Ginger L Milne; Jason D Morrow; Oliver Boutaud; John A Oates
Journal:  J Gastroenterol       Date:  2009-01-16       Impact factor: 7.527

4.  Mechanism of inhibition of human secretory phospholipase A2 by flavonoids: rationale for lead design.

Authors:  Jens Lättig; Markus Böhl; Petra Fischer; Sandra Tischer; Claudia Tietböhl; Mario Menschikowski; Herwig O Gutzeit; Peter Metz; M Teresa Pisabarro
Journal:  J Comput Aided Mol Des       Date:  2007-08-15       Impact factor: 3.686

5.  On the role of protein disulfide isomerase in the retrograde cell transport of secreted phospholipases A2.

Authors:  Jernej Oberčkal; Lidija Kovačič; Jernej Šribar; Adrijana Leonardi; Klemen Dolinar; Anja Pucer Janež; Igor Križaj
Journal:  PLoS One       Date:  2015-03-12       Impact factor: 3.240

6.  Psoriatic T cells recognize neolipid antigens generated by mast cell phospholipase delivered by exosomes and presented by CD1a.

Authors:  Ka Lun Cheung; Rachael Jarrett; Sumithra Subramaniam; Maryam Salimi; Danuta Gutowska-Owsiak; Yi-Ling Chen; Clare Hardman; Luzheng Xue; Vincenzo Cerundolo; Graham Ogg
Journal:  J Exp Med       Date:  2016-09-26       Impact factor: 14.307
  6 in total

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