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Literature DB >> 15173352

High-throughput polyribosome fractionation.

Yipeng Wang¹, Steven Ringquist, Ann H Cho, Gaelle Rondeau, John Welsh.

Abstract

Polyribosome sedimentation velocity centrifugation can be used to identify differential regulation of the translation of mRNAs. However, ultracentrifugation presents practical limitations on the number of sedimentation velocity gradients that can be run simultaneously. A method for sedimentation velocity analysis of polyribosomes is presented that is based on low-speed centrifugation of sucrose gradients prepared in deep 96-well plates, the advantage of which is that hundreds of polyribosome fractionations can be performed simultaneously in a tabletop centrifuge.

Entities: Chemical

Mesh：

Substances：
RNA, Messenger
Puromycin

Year: 2004 PMID： 15173352 PMCID： PMC434456 DOI： 10.1093/nar/gnh077

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

22 in total

1. Genome-wide analysis of mRNA translation profiles in Saccharomyces cerevisiae.

Authors: Yoav Arava; Yulei Wang; John D Storey; Chih Long Liu; Patrick O Brown; Daniel Herschlag
Journal: Proc Natl Acad Sci U S A Date: 2003-03-26 Impact factor: 11.205

2. Promoter choice influences alternative splicing and determines the balance of isoforms expressed from the mouse bcl-X gene.

Authors: A Pecci; L R Viegas; J L Baranao; M Beato
Journal: J Biol Chem Date: 2001-03-26 Impact factor: 5.157

3. Oncogenic Ras and Akt signaling contribute to glioblastoma formation by differential recruitment of existing mRNAs to polysomes.

Authors: Vinagolu K Rajasekhar; Agnes Viale; Nicholas D Socci; Martin Wiedmann; Xiaoyi Hu; Eric C Holland
Journal: Mol Cell Date: 2003-10 Impact factor: 17.970

4. SYNTHESIS OF NASCENT PROTEIN BY RIBOSOMES IN ESCHERICHIA COLI.

Authors: K McQuillen; R B Roberts; R J Britten
Journal: Proc Natl Acad Sci U S A Date: 1959-09 Impact factor: 11.205

5. Coordinate regulation of transcription and splicing by steroid receptor coregulators.

Authors: Didier Auboeuf; Arnd Hönig; Susan M Berget; Bert W O'Malley
Journal: Science Date: 2002-10-11 Impact factor: 47.728

6. von Hippel-Lindau protein-mediated repression of tumor necrosis factor alpha translation revealed through use of cDNA arrays.

Authors: Stefanie Galbán; Jinshui Fan; Jennifer L Martindale; Chris Cheadle; Bryan Hoffman; Michael P Woods; Gretchen Temeles; Jürgen Brieger; Jochen Decker; Myriam Gorospe
Journal: Mol Cell Biol Date: 2003-04 Impact factor: 4.272

7. The role of the 3' untranslated region in mRNA sorting to the vicinity of mitochondria is conserved from yeast to human cells.

Authors: J Sylvestre; A Margeot; C Jacq; G Dujardin; M Corral-Debrinski
Journal: Mol Biol Cell Date: 2003-06-13 Impact factor: 4.138

High-throughput polyribosome fractionation.

1. Genome-wide analysis of mRNA translation profiles in Saccharomyces cerevisiae.

2. Promoter choice influences alternative splicing and determines the balance of isoforms expressed from the mouse bcl-X gene.

3. Oncogenic Ras and Akt signaling contribute to glioblastoma formation by differential recruitment of existing mRNAs to polysomes.

4. SYNTHESIS OF NASCENT PROTEIN BY RIBOSOMES IN ESCHERICHIA COLI.

5. Coordinate regulation of transcription and splicing by steroid receptor coregulators.

6. von Hippel-Lindau protein-mediated repression of tumor necrosis factor alpha translation revealed through use of cDNA arrays.

7. The role of the 3' untranslated region in mRNA sorting to the vicinity of mitochondria is conserved from yeast to human cells.

8. Influence of the RNA-binding protein HuR in pVHL-regulated p53 expression in renal carcinoma cells.

9. An eIF4AIII-containing complex required for mRNA localization and nonsense-mediated mRNA decay.

10. Decreased proliferation of human melanoma cell lines caused by antisense RNA against translation factor eIF-4A1.

1. Ribosome queuing enables non-AUG translation to be resistant to multiple protein synthesis inhibitors.

2. Assessment of selective mRNA translation in mammalian cells by polysome profiling.

3. Identifying the Translatome of Mouse NEBD-Stage Oocytes via SSP-Profiling; A Novel Polysome Fractionation Method.