Literature DB >> 1517171

Diagnostic application of monoclonal antibodies to outer membrane protein for rapid detection of Salmonella.

S Kerr1, H J Ball, D P Mackie, D A Pollock, D A Finlay.   

Abstract

Monoclonal antibodies produced to Salmonella enteritidis outer membrane proteins were screened against 57 Salmonella serovars and several related enterobacteria. Those detecting all Salmonella serovars and none of the related enterobacteria were used in a microtitre plate antigen capture ELISA to screen clinical samples. Sixty-one of 2100 samples yielded salmonellas after incubation for 24 h in selective media by conventional culture. Of these 58 were detected by the ELISA. Sixty-five false positives by ELISA were found to be Enterobacter spp. The results show the potential of this ELISA to eliminate a large proportion of the salmonella-negative cultures at an early stage.

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Year:  1992        PMID: 1517171     DOI: 10.1111/j.1365-2672.1992.tb01839.x

Source DB:  PubMed          Journal:  J Appl Bacteriol        ISSN: 0021-8847


  2 in total

1.  A repertoire of high-affinity monoclonal antibodies specific to S. typhi: as potential candidate for improved typhoid diagnostic.

Authors:  Chandresh Sharma; Anurag Sankhyan; Tarang Sharma; Naeem Khan; Susmita Chaudhuri; Niraj Kumar; Shinjini Bhatnagar; Navin Khanna; Ashutosh Tiwari
Journal:  Immunol Res       Date:  2015-07       Impact factor: 2.829

2.  Antigenic relationships within the genus Salmonella as revealed by anti-Salmonella enteritidis monoclonal antibodies.

Authors:  M Malik; G Butchaiah; M P Bansal; M Z Siddiqui; C S Bakshi; R K Singh
Journal:  Vet Res Commun       Date:  2002-04       Impact factor: 2.459

  2 in total

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