BACKGROUND: Leukotriene-like immunoreactivity has been detected in exhaled breath condensate (EBC), but definitive evidence for the presence of leukotrienes (LTs) in this biological fluid is not available. A study was undertaken to determine whether LTC(4), LTD(4), LTE(4), and LTB(4) are measurable in EBC by gas chromatography/mass spectrometry and to quantify exhaled LTs in adults and children with asthma and in control subjects. METHODS: Twenty eight adults and 33 children with mild to moderate persistent asthma treated with inhaled corticosteroids and age matched healthy controls (50 adults and 50 children) were studied. LTB(4), LTC(4), LTD(4), and LTE(4) in EBC were measured by gas chromatography/mass spectrometry. RESULTS: LTD(4), LTE(4), and LTB(4) were detectable in all samples. Concentrations of LTC(4) in EBC were either close to or below the detection limit of 1 pg/ml. Median exhaled LTD(4), LTE(4), and LTB(4) concentrations in asthmatic adults were increased 4.1-fold (p<0.001), 1.8-fold (p<0.01), and 2.6-fold (p<0.001), respectively, compared with values in healthy adults. Median exhaled LTD(4), LTE(4), and LTB(4) concentrations in asthmatic children were increased 2.8-fold (p<0.001), 1.3-fold (p<0.001), and 1.6-fold (p<0.001), respectively, compared with those in healthy children. In patients with asthma there was a correlation between exhaled LTD(4) and LTE(4) in both adults (r = 0.87, p<0.0001) and children (r = 0.78, p<0.0001). CONCLUSIONS: Gas chromatography/mass spectrometry can be used to accurately quantify exhaled LTs which are increased in asthmatic adults and children compared with controls.
BACKGROUND:Leukotriene-like immunoreactivity has been detected in exhaled breath condensate (EBC), but definitive evidence for the presence of leukotrienes (LTs) in this biological fluid is not available. A study was undertaken to determine whether LTC(4), LTD(4), LTE(4), and LTB(4) are measurable in EBC by gas chromatography/mass spectrometry and to quantify exhaled LTs in adults and children with asthma and in control subjects. METHODS: Twenty eight adults and 33 children with mild to moderate persistent asthma treated with inhaled corticosteroids and age matched healthy controls (50 adults and 50 children) were studied. LTB(4), LTC(4), LTD(4), and LTE(4) in EBC were measured by gas chromatography/mass spectrometry. RESULTS: LTD(4), LTE(4), and LTB(4) were detectable in all samples. Concentrations of LTC(4) in EBC were either close to or below the detection limit of 1 pg/ml. Median exhaled LTD(4), LTE(4), and LTB(4) concentrations in asthmatic adults were increased 4.1-fold (p<0.001), 1.8-fold (p<0.01), and 2.6-fold (p<0.001), respectively, compared with values in healthy adults. Median exhaled LTD(4), LTE(4), and LTB(4) concentrations in asthmatic children were increased 2.8-fold (p<0.001), 1.3-fold (p<0.001), and 1.6-fold (p<0.001), respectively, compared with those in healthy children. In patients with asthma there was a correlation between exhaled LTD(4) and LTE(4) in both adults (r = 0.87, p<0.0001) and children (r = 0.78, p<0.0001). CONCLUSIONS: Gas chromatography/mass spectrometry can be used to accurately quantify exhaled LTs which are increased in asthmatic adults and children compared with controls.
Authors: Richard M Effros; Julie Biller; Bradley Foss; Kelly Hoagland; Marshall B Dunning; Daniel Castillo; Mark Bosbous; Feng Sun; Reza Shaker Journal: Am J Respir Crit Care Med Date: 2003-09-25 Impact factor: 21.405
Authors: S E Wenzel; J B Trudeau; D A Kaminsky; J Cohn; R J Martin; J Y Westcott Journal: Am J Respir Crit Care Med Date: 1995-09 Impact factor: 21.405
Authors: K Asano; C M Lilly; W J O'Donnell; E Israel; A Fischer; B J Ransil; J M Drazen Journal: J Allergy Clin Immunol Date: 1995-11 Impact factor: 10.793
Authors: William Checkley; Maria P Deza; Jost Klawitter; Karina M Romero; Jelena Klawitter; Suzanne L Pollard; Robert A Wise; Uwe Christians; Nadia N Hansel Journal: Respir Med Date: 2016-10-21 Impact factor: 3.415
Authors: T Ichikawa; K Matsunaga; Y Minakata; S Yanagisawa; K Ueshima; K Akamatsu; T Hirano; M Nakanishi; H Sugiura; T Yamagata; M Ichinose Journal: Anal Chem Insights Date: 2007-10-12
Authors: Luis M Gonzalez-Reche; Anita K Musiol; Alice Müller-Lux; Thomas Kraus; Thomas Göen Journal: J Occup Med Toxicol Date: 2006-05-17 Impact factor: 2.646