Literature DB >> 15165516

Immobilized metal ion affinity-based fluorescence polarization (IMAP): advances in kinase screening.

J Richard Sportsman1, Elizabeth A Gaudet, Annegret Boge.   

Abstract

The IMAP Fluorescence Polarization technology is a homogeneous antibody-free method for analysis of kinases, phosphatases, and phosphodiesterases. Recent developments to the technology include an enhancement of the reagent system (the Progressive Binding System) that significantly expands the range of useable concentrations of ATP, choices of substrates, and assay configurations. With the new Progressive System, we are able to design multiplexed assays that allow the simultaneous determination of multiple kinase activities. In addition, coupled assays are now possible, allowing the assay of kinases through natural or artificial coupling through kinase cascades.

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Year:  2004        PMID： 15165516     DOI： 10.1089/154065804323056549

Source DB:  PubMed          Journal:  Assay Drug Dev Technol        ISSN： 1540-658X            Impact factor:   1.738


  14 in total

1.  Assay principle for modulators of protein-protein interactions and its application to non-ATP-competitive ligands targeting protein kinase A.

Authors:  S Adrian Saldanha; Gregory Kaler; Howard B Cottam; Ruben Abagyan; Susan S Taylor
Journal:  Anal Chem       Date:  2006-12-15       Impact factor: 6.986

2.  A basis for reduced chemical library inhibition of firefly luciferase obtained from directed evolution.

Authors:  Douglas S Auld; Ya-Qin Zhang; Noel T Southall; Ganesha Rai; Marc Landsman; Jennifer MacLure; Daniel Langevin; Craig J Thomas; Christopher P Austin; James Inglese
Journal:  J Med Chem       Date:  2009-03-12       Impact factor: 7.446

3.  Comparison of bioluminescent kinase assays using substrate depletion and product formation.

Authors:  Cordelle Tanega; Min Shen; Bryan T Mott; Craig J Thomas; Ryan MacArthur; James Inglese; Douglas S Auld
Journal:  Assay Drug Dev Technol       Date:  2009-12       Impact factor: 1.738

4.  Sensitive measurement of total protein phosphorylation level in complex protein samples.

Authors:  Li Pan; Linna Wang; Chuan-Chih Hsu; Jiazhen Zhang; Anton Iliuk; W Andy Tao
Journal:  Analyst       Date:  2015-04-10       Impact factor: 4.616

5.  Structural and functional analysis of the natural JNK1 inhibitor quercetagetin.

Authors:  Sohee Baek; Nam Joo Kang; Grzegorz M Popowicz; Marcelino Arciniega; Sung Keun Jung; Sanguine Byun; Nu Ry Song; Yong-Seok Heo; Bo Yeon Kim; Hyong Joo Lee; Tad A Holak; Martin Augustin; Ann M Bode; Robert Huber; Zigang Dong; Ki Won Lee
Journal:  J Mol Biol       Date:  2012-11-08       Impact factor: 5.469

6.  Fluorescence polarization assays in small molecule screening.

Authors:  Wendy A Lea; Anton Simeonov
Journal:  Expert Opin Drug Discov       Date:  2011-01       Impact factor: 6.098

7.  Molecular conformations, interactions, and properties associated with drug efficiency and clinical performance among VEGFR TK inhibitors.

Authors:  Michele McTigue; Brion William Murray; Jeffrey H Chen; Ya-Li Deng; James Solowiej; Robert S Kania
Journal:  Proc Natl Acad Sci U S A       Date:  2012-09-17       Impact factor: 11.205

Review 8.  Targeting mutant KRAS for anticancer therapeutics: a review of novel small molecule modulators.

Authors:  Yuanxiang Wang; Christine E Kaiser; Brendan Frett; Hong-Yu Li
Journal:  J Med Chem       Date:  2013-04-23       Impact factor: 7.446

9.  Development and implementation of a miniaturized high-throughput time-resolved fluorescence energy transfer assay to identify small molecule inhibitors of polo-like kinase 1.

Authors:  Elizabeth R Sharlow; Stephanie Leimgruber; Tong Ying Shun; John S Lazo
Journal:  Assay Drug Dev Technol       Date:  2007-12       Impact factor: 1.738

10.  Development of an orally-administrative MELK-targeting inhibitor that suppresses the growth of various types of human cancer.

Authors:  Suyoun Chung; Hanae Suzuki; Takashi Miyamoto; Naofumi Takamatsu; Ayako Tatsuguchi; Koji Ueda; Kyoko Kijima; Yusuke Nakamura; Yo Matsuo
Journal:  Oncotarget       Date:  2012-12
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