| Literature DB >> 15165458 |
Peter Ashjian1, Amir Elbarbary, Patricia Zuk, Daniel A DeUgarte, Prosper Benhaim, Laura Marcu, Marc H Hedrick.
Abstract
The clinical implantation of bioengineered tissues requires an in situ nondestructive evaluation of the quality of tissue constructs developed in vitro before transplantation. Time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) is demonstrated here to noninvasively monitor the formation of osteogenic extracellular matrix (ECM) produced by putative stem cells (PLA cells) derived from human adipose tissue. We show that this optical spectroscopy technique can assess the relative expression of collagens (types I, III, IV, and V) within newly forming osteogenic ECM. The results are consistent with those obtained by conventional histochemical techniques (immunofluorescence and Western blot) and demonstrate that TR-LIFS is a potential tool for monitoring the expression of distinct collagen types and the formation of collagen cross-links in intact tissue constructs.Entities:
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Year: 2004 PMID： 15165458 DOI： 10.1089/107632704323061771
Source DB: PubMed Journal: Tissue Eng ISSN： 1076-3279