Methodological evaluation to analyze functional recovery after sciatic nerve injury.

The Basso, Bresnahan and Beattie (BBB) locomotor scale has not been tested to evaluate functional consequences of peripheral nerve lesions. Alternative methods to evaluate animal functional recovery after sciatic nerve injury are desirable. Male Wistar rats had a right sciatic nerve segment exposed and were divided in three experimental groups: Sham (wound open, 10 min), Sham-device (nerve segment between crushing device, 10 min), and Crush-force (nerve crushing load of 15,000 g/1,000 mm Hg/mm(2), 10 min). Animals were evaluated preoperatively, 1, 7, 14, 21, and 28 days after procedure by calculation of Sciatic Functional Index (SFI), BBB score and open arena exploratory activity. The primary findings of the present study were (1) the SFI calculated by either DeMedinaceli, Carlton and Goldberg, and Bain formulae were highly correlated; (2) the BBB score evaluation was highly correlated with the SFI; (3) the BBB motor scale was able to detect functional impairments not recognized by the SFI; and (4) open arena exploratory activity was a poor method to detect sciatic nerve impairment. In conclusion, the BBB prescribed functional deficits on the sham-device and crush-force groups even when the SFI indicated full recovery. This greater sensitivity may prove useful when comparing new therapeutic approaches to nerve regeneration.