PURPOSE: To identify the genotype of 18S ribosomal RNA gene (18S rDNA, Rns) of the Acanthamoeba strains isolated from patients with keratitis in northern China. METHODS: The genus-specific primers JDP1 and JDP2 were used for the amplification of the amplimer ASA.S1. With DNA PCR and sequencing, Rns genotypes were identified according to the DF3 sequence variances. RESULTS: Of all 26 DF3 sequences obtained from the 26 Acanthamoeba strains, 18 were unique (69.2%). Of those, 17 were Rns genotype T4 and one was Rns genotype T3. CONCLUSIONS: Most Acanthamoeba strains isolated from keratitis in northern China were Rns genotype T4. The results are in agreement with recent results in the literature.
PURPOSE: To identify the genotype of 18S ribosomal RNA gene (18S rDNA, Rns) of the Acanthamoeba strains isolated from patients with keratitis in northern China. METHODS: The genus-specific primers JDP1 and JDP2 were used for the amplification of the amplimer ASA.S1. With DNA PCR and sequencing, Rns genotypes were identified according to the DF3 sequence variances. RESULTS: Of all 26 DF3 sequences obtained from the 26 Acanthamoeba strains, 18 were unique (69.2%). Of those, 17 were Rns genotype T4 and one was Rns genotype T3. CONCLUSIONS: Most Acanthamoeba strains isolated from keratitis in northern China were Rns genotype T4. The results are in agreement with recent results in the literature.
Authors: A Magnet; A L Galván; S Fenoy; F Izquierdo; C Rueda; C Fernandez Vadillo; J Pérez-Irezábal; K Bandyopadhyay; G S Visvesvara; A J da Silva; C del Aguila Journal: Parasitol Res Date: 2012-03-07 Impact factor: 2.289
Authors: D R Ledee; A Iovieno; D Miller; N Mandal; M Diaz; J Fell; M E Fini; E C Alfonso Journal: J Clin Microbiol Date: 2009-03-25 Impact factor: 5.948
Authors: Chandirasegaran Massilamany; Francine Marciano-Cabral; Bruno da Rocha-Azevedo; Melissa Jamerson; Arunakumar Gangaplara; David Steffen; Rana Zabad; Zsolt Illes; Raymond A Sobel; Jay Reddy Journal: PLoS One Date: 2014-05-30 Impact factor: 3.240