BACKGROUND: The purpose of this study was to identify homeobox genes expressed in the human colon and to determine whether their expression levels were altered between matched non-malignant and malignant colon tissues. MATERIALS AND METHODS: Homeobox genes expressed in colon tissue were identified by reverse transcription polymerase chain reaction (RT-PCR). Antibodies were raised to the homeodomain proteins DLX4, HB9 and HB24 and immunohistochemistry was performed on 3 moderately-differentiated tumors and their corresponding non-malignant colon tissue samples. RESULTS: The RT-PCR screen identified expression of DLX4, HB9, HB24 and MSX2 in the normal colon. Immunoaffinity purified polyclonal antisera raised against DLX4, HB9 and HB24 detect specific immunoreactivity in glandular epithelial cells, stromal cells of the lamina propria but not in the submucosa. Nuclear epithelial immunoreactivity of all three antibodies decreased in moderately-differentiated tumors compared to the corresponding matched non-malignant mucosa. These data suggest that differential expression of HB9, HB24 and DLX4 may be associated with colorectal carcinogenesis.
BACKGROUND: The purpose of this study was to identify homeobox genes expressed in the human colon and to determine whether their expression levels were altered between matched non-malignant and malignant colon tissues. MATERIALS AND METHODS: Homeobox genes expressed in colon tissue were identified by reverse transcription polymerase chain reaction (RT-PCR). Antibodies were raised to the homeodomain proteins DLX4, HB9 and HB24 and immunohistochemistry was performed on 3 moderately-differentiated tumors and their corresponding non-malignant colon tissue samples. RESULTS: The RT-PCR screen identified expression of DLX4, HB9, HB24 and MSX2 in the normal colon. Immunoaffinity purified polyclonal antisera raised against DLX4, HB9 and HB24 detect specific immunoreactivity in glandular epithelial cells, stromal cells of the lamina propria but not in the submucosa. Nuclear epithelial immunoreactivity of all three antibodies decreased in moderately-differentiated tumors compared to the corresponding matched non-malignant mucosa. These data suggest that differential expression of HB9, HB24 and DLX4 may be associated with colorectal carcinogenesis.
Authors: Di Wu; Shyamali Mandal; Alex Choi; August Anderson; Michaela Prochazkova; Hazel Perry; Vera L Gil-Da-Silva-Lopes; Richard Lao; Eunice Wan; Paul Ling-Fung Tang; Pui-yan Kwok; Ophir Klein; Bian Zhuan; Anne M Slavotinek Journal: Hum Mol Genet Date: 2015-05-07 Impact factor: 6.150
Authors: Béla Molnár; Orsolya Galamb; Bálint Péterfia; Barnabás Wichmann; István Csabai; András Bodor; Alexandra Kalmár; Krisztina Andrea Szigeti; Barbara Kinga Barták; Zsófia Brigitta Nagy; Gábor Valcz; Árpád V Patai; Péter Igaz; Zsolt Tulassay Journal: BMC Cancer Date: 2018-06-27 Impact factor: 4.430
Authors: Šárka Šestáková; Ela Cerovská; Cyril Šálek; Dávid Kundrát; Ivana Ježíšková; Adam Folta; Jiří Mayer; Zdeněk Ráčil; Petr Cetkovský; Hana Remešová Journal: Clin Epigenetics Date: 2022-02-11 Impact factor: 6.551