BACKGROUND: Enzymatic activity levels of serum CD26/dipeptidylpeptidase (DPP) IV in oral cancer patients are lower than those in healthy subjects. However, the mechanism for this decrease is not yet fully understood. MATERIALS AND METHODS: The influence of malignant cell-derived cytokines on cell surface CD26/DPPIV expression in human T cells was analyzed using an oral squamous cell carcinoma (SCC) cell line, KB, and peripheral blood T cells. RESULTS: Tumor growth factor (TGF)-beta1 in KB-conditioned medium (KBCM) down-regulated CD26/DPPIV expression in T cells, which was responsible for the decreased DPPIV activities in the cultured supernatant. Expression p27kip in T cells was maintained in addition to G1 arrest when cultured with KBCM but was abolished by inclusion of anti-TGF-beta1 antibody. CONCLUSION: SCC-derived TGF-beta1 down-regulates CD26/DPPIV expression in T cells, resulting in decreased serum CD26/DPPIV activity in oral cancer patients.
BACKGROUND: Enzymatic activity levels of serum CD26/dipeptidylpeptidase (DPP) IV in oral cancerpatients are lower than those in healthy subjects. However, the mechanism for this decrease is not yet fully understood. MATERIALS AND METHODS: The influence of malignant cell-derived cytokines on cell surface CD26/DPPIV expression in human T cells was analyzed using an oral squamous cell carcinoma (SCC) cell line, KB, and peripheral blood T cells. RESULTS: Tumor growth factor (TGF)-beta1 in KB-conditioned medium (KBCM) down-regulated CD26/DPPIV expression in T cells, which was responsible for the decreased DPPIV activities in the cultured supernatant. Expression p27kip in T cells was maintained in addition to G1 arrest when cultured with KBCM but was abolished by inclusion of anti-TGF-beta1 antibody. CONCLUSION: SCC-derived TGF-beta1 down-regulates CD26/DPPIV expression in T cells, resulting in decreased serum CD26/DPPIV activity in oral cancerpatients.
Authors: Oscar J Cordero; Monica Imbernon; Loretta De Chiara; Vicenta S Martinez-Zorzano; Daniel Ayude; Maria Paez de la Cadena; F Javier Rodriguez-Berrocal Journal: World J Clin Oncol Date: 2011-06-10
Authors: Oscar J Cordero; Rubén Varela-Calviño; Tania López-González; Cristina Calviño-Sampedro; Juan E Viñuela; Coral Mouriño; Íñigo Hernández-Rodríguez; Marina Rodríguez-López; Bruno Aspe de la Iglesia; José María Pego-Reigosa; José María Pego Journal: PLoS One Date: 2015-07-15 Impact factor: 3.240
Authors: Loretta De Chiara; Ana M Rodríguez-Piñeiro; Oscar J Cordero; Lidia Vázquez-Tuñas; Daniel Ayude; Francisco J Rodríguez-Berrocal; María Páez de la Cadena Journal: PLoS One Date: 2014-09-11 Impact factor: 3.240