| Literature DB >> 15158475 |
Hong Zhang1, Qing Gu, Xinle Liang, Yuanjiang Pan.
Abstract
A new rapid compound screening approach for topoisomerase I binding activity is presented. DNA topoisomerase I is used as a target protein to capture binding compounds from a mixture of combinatorial compounds by bioaffinity ultrafiltration. Using high-performance liquid chromatography combined with electrospray ionization mass spectrometry, small-molecule active compounds were identified. We also have successfully applied this method to identifying compounds from cells grown in culture.Entities:
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Year: 2004 PMID： 15158475 DOI： 10.1016/j.ab.2004.03.057
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365