| Literature DB >> 15157889 |
P T Tran1, Anne Paoletti, Fred Chang.
Abstract
The use of green fluorescent protein (GFP) fusions as biosensors for examining protein localization and dynamics has revolutionized cell biology. Here, we describe the methods developed for imaging of GFP-fusions in the fission yeast Schizosaccharomyces pombe using fluorescence microscopy, with a focus on the use of time-lapse imaging to analyze the dynamics of microtubules. We discuss the considerations in fluorescence microscopy, cell preparation, data acquisition, and image analysis appropriate for analysis of living cells.Entities:
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Year: 2004 PMID： 15157889 DOI： 10.1016/j.ymeth.2003.11.017
Source DB: PubMed Journal: Methods ISSN： 1046-2023 Impact factor: 3.608