AIM: To screen bioactive peptides that mimic the epitope of gastric cancer associated antigen. METHODS: Anti-gastric cancer monoclonal antibody (mAb) MG7 was purified by ion chromatography, and then coated on ELISA plate. By using the mAb as selective molecular, a 12-meres phage displaying peptide library was biopanned, and the positive clones were selected. RESULTS: 12 positive phage clones were selected. CONCLUSION: A candidate mimic epitope of gastric cancer associated antigen was identified, which provided the foundation for developing tumor peptide vaccine.
AIM: To screen bioactive peptides that mimic the epitope of gastric cancer associated antigen. METHODS: Anti-gastric cancer monoclonal antibody (mAb) MG7 was purified by ion chromatography, and then coated on ELISA plate. By using the mAb as selective molecular, a 12-meres phage displaying peptide library was biopanned, and the positive clones were selected. RESULTS: 12 positive phage clones were selected. CONCLUSION: A candidate mimic epitope of gastric cancer associated antigen was identified, which provided the foundation for developing tumor peptide vaccine.
Authors: T Lin; S Liang; F Meng; Q Han; C Guo; L Sun; Y Chen; Z Liu; Z Yu; H Xie; J Ding; D Fan Journal: Clin Exp Immunol Date: 2006-05 Impact factor: 4.330