AIM: To investigate the stage-specific localization of transforming growth factor (TGF) beta1 and beta3 during spermatogenesis in adult human testis. METHODS: The localization of TGFbeta1 and beta3 was investigated by immunohistochemical staining method employing specific polyclonal antibodies. RESULTS: Both TGFbeta1 and beta3 and their receptors were preponderant in the Leydig cells. TGFbeta1 could not be detected in the seminiferous tubules. TGFbeta3 and TGFbeta-Receptor (R) I were mainly seen in the elongated spermatids, while TGFbeta-RII in the pachytene spermatocytes and weak in the spermatogonia, spermatids and Sertoli cells. Only TGFbeta-RII was detected in the Sertoli cells. TGFbeta3, TGFbeta-RI and TGFbeta-RII showed a staining pattern dependent upon the stages of the seminiferous epithelium cycle. CONCLUSION: TGFbeta isoforms and their receptors are present in the somatic and germ cells of the adult human testis, suggesting their involvement in the regulation of spermatogenesis.
AIM: To investigate the stage-specific localization of transforming growth factor (TGF) beta1 and beta3 during spermatogenesis in adult human testis. METHODS: The localization of TGFbeta1 and beta3 was investigated by immunohistochemical staining method employing specific polyclonal antibodies. RESULTS: Both TGFbeta1 and beta3 and their receptors were preponderant in the Leydig cells. TGFbeta1 could not be detected in the seminiferous tubules. TGFbeta3 and TGFbeta-Receptor (R) I were mainly seen in the elongated spermatids, while TGFbeta-RII in the pachytene spermatocytes and weak in the spermatogonia, spermatids and Sertoli cells. Only TGFbeta-RII was detected in the Sertoli cells. TGFbeta3, TGFbeta-RI and TGFbeta-RII showed a staining pattern dependent upon the stages of the seminiferous epithelium cycle. CONCLUSION:TGFbeta isoforms and their receptors are present in the somatic and germ cells of the adult human testis, suggesting their involvement in the regulation of spermatogenesis.
Authors: Pedro P Rojas-García; Mónica P Recabarren; Teresa Sir-Petermann; Rodolfo Rey; Sergio Palma; Albert Carrasco; Carlos C Perez-Marin; Vasantha Padmanabhan; Sergio E Recabarren Journal: Endocrine Date: 2012-10-18 Impact factor: 3.633
Authors: Candela R Gonzalez; María E Matzkin; Mónica B Frungieri; Claudio Terradas; Roberto Ponzio; Elisa Puigdomenech; Oscar Levalle; Ricardo S Calandra; Silvia I Gonzalez-Calvar Journal: Reprod Biol Endocrinol Date: 2010-12-02 Impact factor: 5.211