Literature DB >> 151529

Improved radioenzymatic assay for spectinomycin.

J G Cannon, C P Moran, K F Bott.   

Abstract

The sensitivity and reproducibility of the radioenzymatic adenylylating assay for spectinomycin were improved by modifications of the assay procedure and by partial purification of the enzyme. The presence of Bacillus subtilis ribosomes or S-150 fraction in the enzymatic reaction mixture interfered with the ability to measure spectinomycin by this method.

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Year:  1978        PMID： 151529      PMCID： PMC352428          DOI： 10.1128/AAC.14.2.168

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  7 in total

1.  Preparation of stable gentamicin adenylyl transferase of high specific activity.

Authors:  P R Goldman; D B Northrop
Journal:  Biochem Biophys Res Commun       Date:  1975-10-27       Impact factor: 3.575

2.  Enzymatic assay for gentamicin and related aminoglycoside antibiotics.

Authors:  R K Holmes; J P Sanford
Journal:  J Infect Dis       Date:  1974-05       Impact factor: 5.226

3.  Assay for gentamicin.

Authors:  R C Tilton; J R Murphy; E Mallett
Journal:  N Engl J Med       Date:  1972-11-23       Impact factor: 91.245

4.  Enzymatic acetylation as a means of determining serum aminoglycoside concentrations.

Authors:  M J Haas; J Davies
Journal:  Antimicrob Agents Chemother       Date:  1973-10       Impact factor: 5.191

5.  The problems of drug-resistant pathogenic bacteria. R factors: biochemical mechanisms of resistance to aminoglycoside antibiotics.

Authors:  J Davies; M Brzezinska; R Benveniste
Journal:  Ann N Y Acad Sci       Date:  1971-06-11       Impact factor: 5.691

6.  Enzymatic Adenylylation of Streptomycin and Spectinomycin by R-Factor-Resistant Escherichia coli.

Authors:  R Benveniste; T Yamada; J Davies
Journal:  Infect Immun       Date:  1970-01       Impact factor: 3.441

7.  Natural messenger ribonucleic acid-directed cell-free protein-synthesizing system of Bacillus subtilis.

Authors:  L Legault-Demare; G H Chambliss
Journal:  J Bacteriol       Date:  1974-12       Impact factor: 3.490
  7 in total

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