Literature DB >> 1515143

Improved chemiluminescent western blotting procedure.

I Bronstein1, J C Voyta, O J Murphy, L Bresnick, L J Kricka.   

Abstract

A chemiluminescent Western blotting procedure and its application in assays for human transferrin and human immunodeficiency virus-I antibodies are described. The procedure is based on a chemiluminescent substrate, adamantyl 1,2-dioxetane aryl phosphate and alkaline phosphatase-labeled detection antibodies. Different membranes (polyvinylidene fluoride, nitrocellulose, nylon) and a proprietary membrane treatment agent (Nitro-Block) have been studied. This sensitive blotting procedure utilizing AMPPD, a polyclonal rabbit anti-transferrin:goat anti-rabbit IgG-alkaline phosphatase detection complex, and a PVDF membrane blocked with Nitro-Block permits the detection of 125 pg (1.6 fmol) of human transferrin. A novel 1,2-dioxetane substrate, CSPD, has also been evaluated.

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Year:  1992        PMID： 1515143

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  10 in total

1.  HIV-1 LTR C/EBP binding site sequence configurations preferentially encountered in brain lead to enhanced C/EBP factor binding and increased LTR-specific activity.

Authors:  H L Ross; S Gartner; J C McArthur; J R Corboy; J J McAllister; S Millhouse; B Wigdahl
Journal:  J Neurovirol       Date:  2001-06       Impact factor: 2.643

2.  Induction of antibodies in guinea pigs and rhesus monkeys against the human immunodeficiency virus type 1 envelope: neutralization of nonpathogenic and pathogenic primary isolate simian/human immunodeficiency virus strains.

Authors:  H X Liao; B Etemad-Moghadam; D C Montefiori; Y Sun; J Sodroski; R M Scearce; R W Doms; J R Thomasch; S Robinson; N L Letvin; B F Haynes
Journal:  J Virol       Date:  2000-01       Impact factor: 5.103

3.  The transcription factor C/EBP-beta and its role in ovarian function; evidence for direct involvement in the ovulatory process.

Authors:  M Pall; P Hellberg; M Brännström; M Mikuni; C M Peterson; K Sundfeldt; B Nordén; L Hedin; S Enerbäck
Journal:  EMBO J       Date:  1997-09-01       Impact factor: 11.598

4.  Structural and functional evolution of human immunodeficiency virus type 1 long terminal repeat CCAAT/enhancer binding protein sites and their use as molecular markers for central nervous system disease progression.

Authors:  Tricia H Hogan; Devin L Stauff; Fred C Krebs; Suzanne Gartner; Shane J Quiterio; Brian Wigdahl
Journal:  J Neurovirol       Date:  2003-02       Impact factor: 2.643

5.  Parasite-mediated nuclear factor kappaB regulation in lymphoproliferation caused by Theileria parva infection.

Authors:  G H Palmer; J Machado; P Fernandez; V Heussler; T Perinat; D A Dobbelaere
Journal:  Proc Natl Acad Sci U S A       Date:  1997-11-11       Impact factor: 11.205

6.  Interaction between CCAAT/enhancer binding protein and cyclic AMP response element binding protein 1 regulates human immunodeficiency virus type 1 transcription in cells of the monocyte/macrophage lineage.

Authors:  H L Ross; M R Nonnemacher; T H Hogan; S J Quiterio; A Henderson; J J McAllister; F C Krebs; B Wigdahl
Journal:  J Virol       Date:  2001-02       Impact factor: 5.103

7.  Association of elevated lipoprotein(a) levels and coronary heart disease in NIDDM patients. Relationship with apolipoprotein(a) phenotypes.

Authors:  J Ruiz; J Thillet; T Huby; R W James; D Erlich; P Flandre; P Froguel; J Chapman; P Passa
Journal:  Diabetologia       Date:  1994-06       Impact factor: 10.122

8.  Quantitative analysis of protein phosphorylations and interactions by multi-colour IP-FCM as an input for kinetic modelling of signalling networks.

Authors:  Sumit Deswal; Anna K Schulze; Thomas Höfer; Wolfgang W A Schamel
Journal:  PLoS One       Date:  2011-07-29       Impact factor: 3.240

9.  Role of cyclooxygenase-2 for fluid secretion by the inflamed gallbladder mucosa.

Authors:  B Nilsson; D Delbro; L Hedin; S Friman; S Andius; J Svanvik
Journal:  J Gastrointest Surg       Date:  1998 May-Jun       Impact factor: 3.267

10.  Receptor tyrosine kinase signaling required for integrin alpha v beta 5-directed cell motility but not adhesion on vitronectin.

Authors:  R L Klemke; M Yebra; E M Bayna; D A Cheresh
Journal:  J Cell Biol       Date:  1994-11       Impact factor: 10.539
  10 in total

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