BACKGROUND: trans-Resveratrol, or 3,5,4'trihydroxy-trans-stilbene, is a polyphenolic compound that seems to provide a protective effect against several types of cancer, notably breast cancer. Through its phytoestrogenic properties it regulates the expression of hormone-dependent genes, such as the oncosuppressor BRCA1, in breast cells. This gene is involved in the majority of hereditary breast cancer, as well as sporadic cancers. METHODS: We used three human breast tumor cell lines (HBL100, MCF7 and MBA-MB-231) and one breast cell line (MCF10a) derived from a fibrocystic disease to study in vitro the effect of resveratrol on the transcription of a group of genes whose proteins interact in different pathways with BRCA1. BRCA1, BRCA2, ER alpha, ER beta, p53, p21(waf1/cip1), CBP/P300, RAD51, pS2 and Ki67 mRNA were quantified using real-time quantitative RT-PCR with an ABI 7700 apparatus. RESULTS: Resveratrol modulated the expression of these genes in a pattern dependent on the status of alpha and beta estrogen receptors. These results show that resveratrol regulates gene expression via the estrogen receptor pathway and also an undetermined pathway. CONCLUSION: Thus, resveratrol seems to have an effect on breast tumor cell lines, on a fibrocystic cell line by affecting several factors regulating the function of BRCA1. Copyright 2004 Elsevier B.V.
BACKGROUND: trans-Resveratrol, or 3,5,4'trihydroxy-trans-stilbene, is a polyphenolic compound that seems to provide a protective effect against several types of cancer, notably breast cancer. Through its phytoestrogenic properties it regulates the expression of hormone-dependent genes, such as the oncosuppressor BRCA1, in breast cells. This gene is involved in the majority of hereditary breast cancer, as well as sporadic cancers. METHODS: We used three humanbreast tumor cell lines (HBL100, MCF7 and MBA-MB-231) and one breast cell line (MCF10a) derived from a fibrocystic disease to study in vitro the effect of resveratrol on the transcription of a group of genes whose proteins interact in different pathways with BRCA1. BRCA1, BRCA2, ER alpha, ER beta, p53, p21(waf1/cip1), CBP/P300, RAD51, pS2 and Ki67 mRNA were quantified using real-time quantitative RT-PCR with an ABI 7700 apparatus. RESULTS:Resveratrol modulated the expression of these genes in a pattern dependent on the status of alpha and beta estrogen receptors. These results show that resveratrol regulates gene expression via the estrogen receptor pathway and also an undetermined pathway. CONCLUSION: Thus, resveratrol seems to have an effect on breast tumor cell lines, on a fibrocystic cell line by affecting several factors regulating the function of BRCA1. Copyright 2004 Elsevier B.V.
Authors: Jessica Dennis; Parviz Ghadirian; Julian Little; Jan Lubinski; Jacek Gronwald; Charmaine Kim-Sing; William Foulkes; Pal Moller; Henry T Lynch; Susan L Neuhausen; Susan Domchek; Susan Armel; Claudine Isaacs; Nadine Tung; Kevin Sweet; Peter Ainsworth; Ping Sun; Daniel Krewski; Steven Narod Journal: Breast Date: 2010-06-12 Impact factor: 4.380
Authors: Andreas J Papoutsis; Sarah D Lamore; Georg T Wondrak; Ornella I Selmin; Donato F Romagnolo Journal: J Nutr Date: 2010-07-14 Impact factor: 4.798
Authors: Sidney W Fu; Saurabh P Kirolikar; Erika Ginsburg; Xiaohui Tan; Arnold Schwartz; Samuel J Simmens; Yan-Gao Man; Joseph J Pinzone; Christine Teal; Sanket Awate; Barbara K Vonderhaar; Patricia E Berg Journal: Oncotarget Date: 2016-08-16