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Literature DB >> 1514691

Immunodetection of biotinylated lymphocyte-surface proteins by enhanced chemiluminescence: a nonradioactive method for cell-surface protein analysis.

T Meier¹, S Arni, S Malarkannan, M Poincelet, D Hoessli.

Abstract

Biotinylation and radioiodination have been compared for labeling lymphocyte-surface proteins and the labeled proteins symmetrically immunoprecipitated with antibodies recognizing major lymphocyte markers such as the murine Thy-1, CD25 (the alpha subunit of the interleukin-2 receptor), CD45, and human CD2 glycoproteins. The detection of biotinylated proteins by enhanced chemiluminescence after transfer to nitrocellulose was found to be fast and as efficient as the detection of iodinated proteins by autoradiography. The vectoriality of cell-surface biotinylation was ascertained by two-dimensional electrophoresis of the cellular extract in which the major cytoplasmic proteins were not found biotinylated. This nonradioactive labeling procedure offers a convenient and efficient alternative to radiolabeling of cell surfaces for the biochemical analysis of extracellular domains of membrane proteins.

Entities: Gene Species

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Year: 1992 PMID： 1514691 DOI： 10.1016/0003-2697(92)90165-4

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

16 in total

1. Characterization of MAX.3 antigen, a glycoprotein expressed on mature macrophages, dendritic cells and blood platelets: identity with CD84.

Authors: S W Krause; M Rehli; S Heinz; R Ebner; R Andreesen
Journal: Biochem J Date: 2000-03-15 Impact factor: 3.857

2. The expression of acidic ribosomal phosphoproteins on the surface membrane of different tissues in autoimmune and normal mice which are the target molecules for anti-double-stranded DNA antibodies.

Authors: K H Sun; W T Liu; S J Tang; C Y Tsai; S C Hsieh; T H Wu; S H Han; C L Yu
Journal: Immunology Date: 1996-03 Impact factor: 7.397

3. Mechanical stretch augments PDGF receptor beta expression and protein tyrosine phosphorylation in pulmonary artery tissue and smooth muscle cells.

Authors: Y Tanabe; M Saito; A Ueno; M Nakamura; K Takeishi; K Nakayama
Journal: Mol Cell Biochem Date: 2000-12 Impact factor: 3.396

4. Identification of specific recognition molecules on murine mononuclear phagocytes and B lymphocytes for Vi capsular polysaccharide: modulation of MHC class II expression on stimulation with the polysaccharide.

Authors: A Qadri
Journal: Immunology Date: 1997-09 Impact factor: 7.397

5. Human immunodeficiency virus type 1 membrane fusion mediated by a laboratory-adapted strain and a primary isolate analyzed by resonance energy transfer.

Authors: V Litwin; K A Nagashima; A M Ryder; C H Chang; J M Carver; W C Olson; M Alizon; K W Hasel; P J Maddon; G P Allaway
Journal: J Virol Date: 1996-09 Impact factor: 5.103

6. Vi polysaccharide of Salmonella typhi targets the prohibitin family of molecules in intestinal epithelial cells and suppresses early inflammatory responses.

Authors: Amita Sharma; Ayub Qadri
Journal: Proc Natl Acad Sci U S A Date: 2004-12-02 Impact factor: 11.205

Immunodetection of biotinylated lymphocyte-surface proteins by enhanced chemiluminescence: a nonradioactive method for cell-surface protein analysis.

1. Characterization of MAX.3 antigen, a glycoprotein expressed on mature macrophages, dendritic cells and blood platelets: identity with CD84.

2. The expression of acidic ribosomal phosphoproteins on the surface membrane of different tissues in autoimmune and normal mice which are the target molecules for anti-double-stranded DNA antibodies.

3. Mechanical stretch augments PDGF receptor beta expression and protein tyrosine phosphorylation in pulmonary artery tissue and smooth muscle cells.

4. Identification of specific recognition molecules on murine mononuclear phagocytes and B lymphocytes for Vi capsular polysaccharide: modulation of MHC class II expression on stimulation with the polysaccharide.

5. Human immunodeficiency virus type 1 membrane fusion mediated by a laboratory-adapted strain and a primary isolate analyzed by resonance energy transfer.

6. Vi polysaccharide of Salmonella typhi targets the prohibitin family of molecules in intestinal epithelial cells and suppresses early inflammatory responses.

7. Detection of a LFA-1-like epitope on the surface of erythrocytes infected with a strain of Plasmodium falciparum.

8. Identification of cell surface molecules that interact with pseudorabies virus.

9. Characterization of a nitrogen-regulated protein identified by cell surface biotinylation of a marine phytoplankton.

10. Affinity purification of plasma membranes.