| Literature DB >> 15141948 |
Libo Shan1, Hye-sook Oh, Jianfu Chen, Ming Guo, Jianmin Zhou, James R Alfano, Alan Collmer, Xu Jia, Xiaoyan Tang.
Abstract
Type III secretion systems are highly conserved among gram-negative plant and animal pathogenic bacteria. Through the type III secretion system, bacteria inject a number of virulence proteins into the host cells. Analysis of the whole genome sequence of Pseudomonas syringae pv. tomato DC3000 strain identified a locus, named HopPtoF, that is homologous to the avirulence gene locus avrPphF in P. syringae pv. phaseolicola. The HopPtoF locus harbors two genes, ShcF(Pto) and HopF(Pto), that are preceded by a single hrp box promoter. We present evidence here to show that ShcF(Pto) and HopF(Pto) encode a type III chaperone and a cognate effector, respectively. ShcF(Pto) interacts with and stabilizes the HopF(Pto) protein in the bacterial cell. Translation of HopF(Pto) starts at a rare initiation codon ATA that limits the synthesis of the HopF(Pto) protein to a low level in bacterial cells.Entities:
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Year: 2004 PMID： 15141948 DOI： 10.1094/MPMI.2004.17.5.447
Source DB: PubMed Journal: Mol Plant Microbe Interact ISSN： 0894-0282 Impact factor: 4.171