AIMS: To determine the prevalence of enterotoxin-producing Staphylococcus intermedius in dogs and pigeons. METHODS AND RESULTS: A total of 106 S. intermedius isolates from 44 dogs and 62 pigeons were tested for the production of enterotoxins A, B, C and D by reverse passive latex agglutination (RPLA) and for sec-canine by PCR. Only one isolate from dog was positive for SEC and sec-canine. Screening of sec-canine-negative strains by nested PCR led to the identification of a novel enterotoxin-related gene, se-int. SE-int showed a significant homology (59-61% identity) with SEC and (56.6% identity) SEB. All 44 isolates from dogs and five isolates (8.1%) from pigeons were se-int positive. CONCLUSIONS: While S. intermedius was isolated more frequently from pigeons than from dogs, se-int was more prevalent among the S. intermedius isolates from dogs, compared with the pigeon isolates. SIGNIFICANCE AND IMPACT OF THE STUDY: Further characterization of the se-int-positive S. intermedius strains should clarify their pathogenic potential including enterotoxigenicity and zoonotic transmissibility to human beings.
AIMS: To determine the prevalence of enterotoxin-producing Staphylococcus intermedius in dogs and pigeons. METHODS AND RESULTS: A total of 106 S. intermedius isolates from 44 dogs and 62 pigeons were tested for the production of enterotoxins A, B, C and D by reverse passive latex agglutination (RPLA) and for sec-canine by PCR. Only one isolate from dog was positive for SEC and sec-canine. Screening of sec-canine-negative strains by nested PCR led to the identification of a novel enterotoxin-related gene, se-int. SE-int showed a significant homology (59-61% identity) with SEC and (56.6% identity) SEB. All 44 isolates from dogs and five isolates (8.1%) from pigeons were se-int positive. CONCLUSIONS: While S. intermedius was isolated more frequently from pigeons than from dogs, se-int was more prevalent among the S. intermedius isolates from dogs, compared with the pigeon isolates. SIGNIFICANCE AND IMPACT OF THE STUDY: Further characterization of the se-int-positive S. intermedius strains should clarify their pathogenic potential including enterotoxigenicity and zoonotic transmissibility to human beings.
Authors: Nouri L Ben Zakour; Jeanette Bannoehr; Adri H M van den Broek; Keith L Thoday; J Ross Fitzgerald Journal: J Bacteriol Date: 2011-03-11 Impact factor: 3.490
Authors: Paweł Kwiatkowski; Aleksandra Tabiś; Karol Fijałkowski; Helena Masiuk; Łukasz Łopusiewicz; Agata Pruss; Monika Sienkiewicz; Marcin Wardach; Mateusz Kurzawski; Sebastian Guenther; Jacek Bania; Barbara Dołęgowska; Iwona Wojciechowska-Koszko Journal: Int J Mol Sci Date: 2022-06-06 Impact factor: 6.208
Authors: Nouri L Ben Zakour; Scott A Beatson; Adri H M van den Broek; Keith L Thoday; J Ross Fitzgerald Journal: Front Cell Infect Microbiol Date: 2012-04-18 Impact factor: 5.293