Literature DB >> 15135508

Diagnostic multiplex PCR for toxin genotyping of Clostridium perfringens isolates.

Christoph G Baums1, Ulrich Schotte, Gunter Amtsberg, Ralph Goethe.   

Abstract

In this study we provide a protocol for genotyping Clostridium perfringens with a new multiplex PCR. This PCR enables reliable and specific detection of the toxin genes cpa, cpb, etx, iap, cpe and cpb2 from heat lysed bacterial suspensions. The efficiency of the protocol was demonstrated by typing C. perfringens reference strains and isolates from veterinary bacteriological routine diagnostic specimens.

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Year:  2004        PMID： 15135508     DOI： 10.1016/S0378-1135(03)00126-3

Source DB:  PubMed          Journal:  Vet Microbiol        ISSN： 0378-1135            Impact factor:   3.293


  45 in total

1.  Intestinal lesions in dogs with acute hemorrhagic diarrhea syndrome associated with netF-positive Clostridium perfringens type A.

Authors:  Miriam Leipig-Rudolph; Kathrin Busch; John F Prescott; Iman Mehdizadeh Gohari; Christian M Leutenegger; Walter Hermanns; Georg Wolf; Katrin Hartmann; Jutta Verspohl; Stefan Unterer
Journal:  J Vet Diagn Invest       Date:  2018-04-06       Impact factor: 1.279

2.  Detection of pathogenic clostridia in biogas plant wastes.

Authors:  Jürgen Neuhaus; Awad A Shehata; Monika Krüger
Journal:  Folia Microbiol (Praha)       Date:  2014-07-02       Impact factor: 2.099

3.  Antimicrobial susceptibility of Clostridium perfringens isolates of bovine, chicken, porcine, and turkey origin from Ontario.

Authors:  Durđa Slavić; Patrick Boerlin; Marta Fabri; Kim C Klotins; Jennifer K Zoethout; Pat E Weir; Debbie Bateman
Journal:  Can J Vet Res       Date:  2011-04       Impact factor: 1.310

4.  Prevalence and toxin type of Clostridium perfringens in beef from four different types of meat markets in Seoul, Korea.

Authors:  Dana Jeong; Dong-Hyeon Kim; Il-Byeong Kang; Jung-Whan Chon; Hyunsook Kim; Ae-Son Om; Joo-Yeon Lee; Jin-San Moon; Deog-Hwan Oh; Kun-Ho Seo
Journal:  Food Sci Biotechnol       Date:  2017-04-30       Impact factor: 2.391

5.  Detection and characterization of Clostridium perfringens in the feces of healthy and diarrheic dogs.

Authors:  Michael R Goldstein; Stephen A Kruth; Alexa M E Bersenas; Marie K Holowaychuk; J Scott Weese
Journal:  Can J Vet Res       Date:  2012-07       Impact factor: 1.310

6.  Freshwater suspended sediments and sewage are reservoirs for enterotoxin-positive Clostridium perfringens.

Authors:  Sabrina R Mueller-Spitz; Lisa B Stewart; J Val Klump; Sandra L McLellan
Journal:  Appl Environ Microbiol       Date:  2010-06-25       Impact factor: 4.792

7.  Farm Stage, Bird Age, and Body Site Dominantly Affect the Quantity, Taxonomic Composition, and Dynamics of Respiratory and Gut Microbiota of Commercial Layer Chickens.

Authors:  John M Ngunjiri; Kara J M Taylor; Michael C Abundo; Hyesun Jang; Mohamed Elaish; Mahesh Kc; Amir Ghorbani; Saranga Wijeratne; Bonnie P Weber; Timothy J Johnson; Chang-Won Lee
Journal:  Appl Environ Microbiol       Date:  2019-04-18       Impact factor: 4.792

8.  Sialidase production and genetic diversity in Clostridium perfringens type A isolated from chicken with necrotic enteritis in Brazil.

Authors:  Luis A Llanco; Viviane Nakano; Mario J Avila-Campos
Journal:  Curr Microbiol       Date:  2014-11-06       Impact factor: 2.188

9.  The effect of glyphosate on potential pathogens and beneficial members of poultry microbiota in vitro.

Authors:  Awad A Shehata; Wieland Schrödl; Alaa A Aldin; Hafez M Hafez; Monika Krüger
Journal:  Curr Microbiol       Date:  2012-12-09       Impact factor: 2.188

10.  Quantitative microbial risk assessment for Clostridium perfringens foodborne illness following consumption of kimchi in South Korea.

Authors:  Yukyung Choi; Joohyun Kang; Yewon Lee; Yeongeun Seo; Heeyoung Lee; Sejeong Kim; Jeeyeon Lee; Jimyeong Ha; Hyemin Oh; Yujin Kim; Kye-Hwan Byun; Sang-Do Ha; Yohan Yoon
Journal:  Food Sci Biotechnol       Date:  2020-04-15       Impact factor: 2.391
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