| Literature DB >> 15135406 |
Carolina W Galvão1, Fábio O Pedrosa, Emanuel M Souza, M Geoffrey Yates, Leda S Chubatsu, Maria Berenice R Steffens.
Abstract
The Herbaspirillum seropedicae RecX protein participates in the SOS response: a process in which the RecA protein plays a central role. The RecX protein of the H. seropedicae, fused to a His-tag sequence (RecX His-tagged), was over-expressed in Escherichia coli and purified by metal-affinity chromatography to yield a highly purified and active protein. DNA band-shift assays showed that the RecX His-tagged protein bound to both circular and linear double-stranded DNA and also to circular single-stranded DNA. The apparent affinity of RecX for DNA decreased in the presence of Mg(2+) ions. The ability of RecX to bind DNA may be relevant to its function in the SOS response.Entities:
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Year: 2004 PMID： 15135406 DOI： 10.1016/j.pep.2004.01.014
Source DB: PubMed Journal: Protein Expr Purif ISSN： 1046-5928 Impact factor: 1.650