| Literature DB >> 15134899 |
Rajko Reljic1, Carol Crawford, Stephen Challacombe, Juraj Ivanyi.
Abstract
The beta-galactoside-binding S-type lectin galectin-3/Mac-2, expressed among several other cell types on activated macrophages, is known to bind IgE, but not other Ig classes. We report in this paper that the single major constituent in a detergent lysate from the J774 mouse macrophage cell line bound to a mouse monoclonal IgA-affinity column. This fraction has been identified by mass spectrometry analysis as galectin-3. Binding of both mouse IgA and IgE to galectin-3 coated plates was inhibited by lactose and asialofetuin. Furthermore, three different monoclonal IgAs bound also to purified recombinant hamster galectin-3 coated plates in a concentration dependent manner. The potential functional significance of IgA binding to galectin-3 within macrophages and possibly other cell types is discussed.Entities:
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Year: 2004 PMID： 15134899 DOI： 10.1016/j.imlet.2004.01.015
Source DB: PubMed Journal: Immunol Lett ISSN： 0165-2478 Impact factor: 3.685