Literature DB >> 15131207

Validated 5' nuclease PCR assay for rapid identification of the genus Brucella.

T Bogdanovich1, M Skurnik, P S Lübeck, P Ahrens, J Hoorfar.   

Abstract

A real-time, genus-specific 5' nuclease PCR assay for amplification of a 322-bp fragment of the per gene was developed for rapid (<2 h) identification of Brucella spp. from agar plates. The assay, including an internal amplification control (116 bp), identified Brucella strains (n = 23) and did not detect non-Brucella strains (n = 174), indicating its usefulness, particularly for laboratories with stringent quality assurance programs.

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Year:  2004        PMID： 15131207      PMCID： PMC404622          DOI： 10.1128/JCM.42.5.2261-2263.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

Review 1.  PCR as a diagnostic tool for brucellosis.

Authors:  Betsy J Bricker
Journal:  Vet Microbiol       Date:  2002-12-20       Impact factor: 3.293

2.  Interlaboratory diagnostic accuracy of a Salmonella specific PCR-based method.

Authors:  Burkhard Malorny; Jeffrey Hoorfar; Marta Hugas; Annet Heuvelink; Patrick Fach; Lüppo Ellerbroek; Cornelia Bunge; Christina Dorn; Reiner Helmuth
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3.  How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing.

Authors:  Jacques Godfroid; Claude Saegerman; Vincent Wellemans; Karl Walravens; Jean-Jacques Letesson; Anne Tibor; Alastair Mc Millan; Steve Spencer; Moëz Sanna; Douwe Bakker; Régis Pouillot; Bruno Garin-Bastuji
Journal:  Vet Microbiol       Date:  2002-12-20       Impact factor: 3.293

4.  Brucellosis in the European Union and Norway at the turn of the twenty-first century.

Authors:  Jacques Godfroid; Annemarie Käsbohrer
Journal:  Vet Microbiol       Date:  2002-12-20       Impact factor: 3.293

Review 5.  Brucella evolution and taxonomy.

Authors:  Edgardo Moreno; Axel Cloeckaert; Ignacio Moriyón
Journal:  Vet Microbiol       Date:  2002-12-20       Impact factor: 3.293

6.  Automated 5' nuclease PCR assay for identification of Salmonella enterica.

Authors:  J Hoorfar; P Ahrens; P Rådström
Journal:  J Clin Microbiol       Date:  2000-09       Impact factor: 5.948

7.  Infection of cattle with Yersinia enterocolitica O:9 a cause of the false positive serological reactions in bovine brucellosis diagnostic tests.

Authors:  V Weynants; A Tibor; P A Denoel; C Saegerman; J Godfroid; P Thiange; J J Letesson
Journal:  Vet Microbiol       Date:  1996-01       Impact factor: 3.293

8.  Conservation of seven genes involved in the biosynthesis of the lipopolysaccharide O-side chain in Brucella spp.

Authors:  A Cloeckaert; M Grayon; J M Verger; J J Letesson; F Godfroid
Journal:  Res Microbiol       Date:  2000-04       Impact factor: 3.992

9.  Real-time detection of Brucella abortus, Brucella melitensis and Brucella suis.

Authors:  R Redkar; S Rose; B Bricker; V DelVecchio
Journal:  Mol Cell Probes       Date:  2001-02       Impact factor: 2.365

10.  Toward an international standard for PCR-based detection of food-borne thermotolerant Campylobacters: assay development and analytical validation.

Authors:  P S Lübeck; P Wolffs; S L W On; P Ahrens; P Rådström; J Hoorfar
Journal:  Appl Environ Microbiol       Date:  2003-09       Impact factor: 4.792
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  8 in total

Review 1.  Review of detection of Brucella spp. by polymerase chain reaction.

Authors:  Wei Ling Yu; Klaus Nielsen
Journal:  Croat Med J       Date:  2010-08       Impact factor: 1.351

2.  Brucella spp. distribution, hosting ruminants from Greece, applying various molecular identification techniques.

Authors:  Aristomenis Katsiolis; Dimitrios K Papadopoulos; Ioannis A Giantsis; Konstantinos Papageorgiou; Antonis Zdragas; Nektarios D Giadinis; Evanthia Petridou
Journal:  BMC Vet Res       Date:  2022-05-27       Impact factor: 2.792

3.  Characterization and biological role of the O-polysaccharide gene cluster of Yersinia enterocolitica serotype O:9.

Authors:  Mikael Skurnik; Marta Biedzka-Sarek; Peter S Lübeck; Tea Blom; José Antonio Bengoechea; Camino Pérez-Gutiérrez; Peter Ahrens; Jeffrey Hoorfar
Journal:  J Bacteriol       Date:  2007-08-10       Impact factor: 3.490

Review 4.  [Mission oriented diagnostic real-time PCR].

Authors:  Herbert Tomaso; Holger C Scholz; Sascha Al Dahouk; Wolf D Splettstoesser; Heinrich Neubauer; Martin Pfeffer; Eberhard Straube
Journal:  Wien Klin Wochenschr       Date:  2007       Impact factor: 2.275

5.  Novel real-time PCR detection assay for Brucella suis.

Authors:  C Hänsel; K Mertens; M C Elschner; F Melzer
Journal:  Vet Rec Open       Date:  2015-04-01

6.  Highly Sensitive Bacteriophage-Based Detection of Brucella abortus in Mixed Culture and Spiked Blood.

Authors:  Kirill V Sergueev; Andrey A Filippov; Mikeljon P Nikolich
Journal:  Viruses       Date:  2017-06-10       Impact factor: 5.048

7.  IS711-based real-time PCR assay as a tool for detection of Brucella spp. in wild boars and comparison with bacterial isolation and serology.

Authors:  Vladimira Hinić; Isabelle Brodard; Andreas Thomann; Milena Holub; Raymond Miserez; Carlos Abril
Journal:  BMC Vet Res       Date:  2009-07-14       Impact factor: 2.741

8.  Amplicon DNA melting analysis for the simultaneous detection of Brucella spp and Mycobacterium tuberculosis complex. Potential use in rapid differential diagnosis between extrapulmonary tuberculosis and focal complications of brucellosis.

Authors:  Rocio Sanjuan-Jimenez; Juan D Colmenero; Pilar Bermúdez; Antonio Alonso; Pilar Morata
Journal:  PLoS One       Date:  2013-03-08       Impact factor: 3.240
  8 in total

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