Literature DB >> 15131202

Comparison of flow cytometric and Alamar Blue tests with the proportional method for testing susceptibility of Mycobacterium tuberculosis to rifampin and isoniazid.

Roberto S Reis1, Ivan Neves, Sergio L S Lourenço, Leila S Fonseca, Maria Cristina S Lourenço.   

Abstract

The performance of flow cytometry and the microplate Alamar Blue assay in determining susceptibility of Mycobacterium tuberculosis was assessed by testing 150 Brazilian isolates. The overall agreement was 97.3 and 98% for isoniazid and 94.7 and 100% for rifampin by flow cytometry and MABA, respectively. This study was entirely done in a developing country.

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Year:  2004        PMID: 15131202      PMCID: PMC404654          DOI: 10.1128/JCM.42.5.2247-2248.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  18 in total

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Authors:  Akos Somoskovi; Qunfeng Song; Judit Mester; Charise Tanner; Yvonne M Hale; Linda M Parsons; Max Salfinger
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

3.  [Measurement of sensitivity of the tuberculous bacillus to antibacillary drugs by the method of proportions. Methodology, resistance criteria, results and interpretation].

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Authors:  M A Espinal; A Laszlo; L Simonsen; F Boulahbal; S J Kim; A Reniero; S Hoffner; H L Rieder; N Binkin; C Dye; R Williams; M C Raviglione
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5.  Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity.

Authors:  J O'Brien; I Wilson; T Orton; F Pognan
Journal:  Eur J Biochem       Date:  2000-09

6.  Improved real-time PCR for rapid detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis clinical isolates.

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Authors:  A Alvarez-Barrientos; J Arroyo; R Cantón; C Nombela; M Sánchez-Pérez
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9.  Detection of isoniazid and rifampin resistance in Mycobacterium tuberculosis strains by single-strand conformation polymorphism analysis and restriction fragment length polymorphism.

Authors:  A Piana; M Orrù; M D Masia; G Sotgiu; E Muresu; A Maida
Journal:  New Microbiol       Date:  2003-10       Impact factor: 2.479

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Authors:  Juan-Carlos Palomino; Anandi Martin; Mirtha Camacho; Humberto Guerra; Jean Swings; Françoise Portaels
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5.  Inter- and intra-assay reproducibility of microplate Alamar blue assay results for isoniazid, rifampicin, ethambutol, streptomycin, ciprofloxacin, and capreomycin drug susceptibility testing of Mycobacterium tuberculosis.

Authors:  Brian Leonard; Jorge Coronel; Mark Siedner; Louis Grandjean; Luz Caviedes; Pilar Navarro; Robert H Gilman; David A J Moore
Journal:  J Clin Microbiol       Date:  2008-08-13       Impact factor: 5.948

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7.  A Rapid Assessing Method of Drug Susceptibility Using Flow Cytometry for Mycobacterium tuberculosis Isolates Resistant to Isoniazid, Rifampin, and Ethambutol.

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Review 9.  Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays.

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10.  The effect of temperature on the viability of human mesenchymal stem cells.

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