| Literature DB >> 15129727 |
Rima Menassa1, Wendy Kennette, Vi Nguyen, Robert Rymerson, Anthony Jevnikar, Jim Brandle.
Abstract
The utility of plants for the production of a wide range of recombinant proteins is now clearly established. However, the challenge remains to produce these proteins at sufficient concentrations for extraction to be economically feasible. In this paper, we have investigated the ability of plant cells to accumulate the human interleukin-10 (IL-10) protein targeted to chloroplasts and mitochondria. We found that IL-10 accumulates in chloroplasts only if a 6 x His tag is added at the C-terminus of the protein. The hexapeptide may provide protection from degradation. Conversely, the IL-10 protein does not accumulate in mitochondria. Analysis of the chloroplast-targeted IL-10 protein revealed only monomeric IL-10 and limited biological activity in in vitro cell assays.Entities:
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Year: 2004 PMID： 15129727 DOI： 10.1016/j.jbiotec.2003.11.009
Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307