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Literature DB >> 15129328

Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos.

Gyulin Hwang¹, Ferenc Müller, M Aziz Rahman, Darren W Williams, Paul J Murdock, K John Pasi, Geoffrey Goldspink, Hamid Farahmand, Norman Maclean.

Abstract

A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.

Entities: Gene Species

Mesh：

Substances：

Year: 2004 PMID： 15129328 DOI： 10.1007/s10126-004-3121-2

Source DB: PubMed Journal: Mar Biotechnol (NY) ISSN： 1436-2228 Impact factor: 3.619

27 in total

1. Copy number related transgene expression and mosaic somatic expression in hemizygous and homozygous transgenic tilapia (Oreochromis niloticus).

Authors: M A Rahman; G L Hwang; S A Razak; F Sohm; N Maclean
Journal: Transgenic Res Date: 2000-12 Impact factor: 2.788

Review 2. Transgenic animal bioreactors in biotechnology and production of blood proteins.

Authors: H Lubon
Journal: Biotechnol Annu Rev Date: 1998

Review 3. Regulation and expression of transgenes in fish -- a review.

Authors: A Iyengar; F Müller; N Maclean
Journal: Transgenic Res Date: 1996-05 Impact factor: 2.788

Review 4. The role of serine proteases in the blood coagulation cascade.

Authors: E W Davie; K Fujikawa; K Kurachi; W Kisiel
Journal: Adv Enzymol Relat Areas Mol Biol Date: 1979

5. Stages of embryonic development of the zebrafish.

Authors: C B Kimmel; W W Ballard; S R Kimmel; B Ullmann; T F Schilling
Journal: Dev Dyn Date: 1995-07 Impact factor: 3.780

6. Recombinant human protein C expression in the milk of transgenic pigs and the effect on endogenous milk immunoglobulin and transferrin levels.

Authors: K E Van Cott; H Lubon; F C Gwazdauskas; J Knight; W N Drohan; W H Velander
Journal: Transgenic Res Date: 2001 Impact factor: 2.788

7. Rational combinatorial chemistry-based selection, synthesis and evaluation of an affinity adsorbent for recombinant human clotting factor VII.

Authors: Paul R Morrill; Geeta Gupta; Kenny Sproule; Don Winzor; Jesper Christensen; Inger Mollerup; Christopher R Lowe
Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2002-07-05 Impact factor: 3.205

8. Transgenic production of a variant of human tissue-type plasminogen activator in goat milk: generation of transgenic goats and analysis of expression.

Authors: K M Ebert; J P Selgrath; P DiTullio; J Denman; T E Smith; M A Memon; J E Schindler; G M Monastersky; J A Vitale; K Gordon
Journal: Biotechnology (N Y) Date: 1991-09

9. Expression and fate of CAT reporter gene microinjected into fertilized medaka (Oryzias latipes) eggs in the form of plasmid DNA, recombinant phage particles and its DNA.

Authors: S S Chong; J R Vielkind
Journal: Theor Appl Genet Date: 1989-09 Impact factor: 5.699

Review 10. Transgenic animal bioreactors.

Authors: L M Houdebine
Journal: Transgenic Res Date: 2000 Impact factor: 2.788

4 in total

1. Enhanced recombinant factor VII expression in Chinese hamster ovary cells by optimizing signal peptides and fed-batch medium.

Authors: Lin Peng; Xiao Yu; Chengyuan Li; Yanfei Cai; Yun Chen; Yang He; Jianfeng Yang; Jian Jin; Huazhong Li
Journal: Bioengineered Date: 2016-04-26 Impact factor: 3.269