BACKGROUND: The reduced ability to activate oral tolerance plays a role in the pathogenesis of some gastrointestinal inflammatory diseases. This activation may reflect a preferential reduction of a T-helper (Th)2- or Th3-type response. In recurrent aphthous ulceration (RAU), genetic and environmental factors may contribute to low tolerance, permitting a cytotoxic reaction against the oral epithelium. The cytokine profile has not permitted the definition of RAU as resulting from enhanced Th1 or Th2 responses. A cDNA microarray study would allow the identification of differentially expressed genes and provide a basis for classification of the immune response. METHODS: The cDNA from 29 samples of aphthae and from 11 samples of normal mucosa from aphthae-free volunteers were hybridized on microarray membranes with 1176 genes. RESULTS: Forty-one differentially expressed genes were identified, and a higher expression level of the Th1 gene cluster in RAU was found. CONCLUSIONS: Microarrays permitted us definition of the gene expression profile of the lesion and identify an increased Th1 activity in RAU lesions.
BACKGROUND: The reduced ability to activate oral tolerance plays a role in the pathogenesis of some gastrointestinal inflammatory diseases. This activation may reflect a preferential reduction of a T-helper (Th)2- or Th3-type response. In recurrent aphthous ulceration (RAU), genetic and environmental factors may contribute to low tolerance, permitting a cytotoxic reaction against the oral epithelium. The cytokine profile has not permitted the definition of RAU as resulting from enhanced Th1 or Th2 responses. A cDNA microarray study would allow the identification of differentially expressed genes and provide a basis for classification of the immune response. METHODS: The cDNA from 29 samples of aphthae and from 11 samples of normal mucosa from aphthae-free volunteers were hybridized on microarray membranes with 1176 genes. RESULTS: Forty-one differentially expressed genes were identified, and a higher expression level of the Th1 gene cluster in RAU was found. CONCLUSIONS: Microarrays permitted us definition of the gene expression profile of the lesion and identify an increased Th1 activity in RAU lesions.
Authors: Bing Ma; Min-Jong Kang; Chun Geun Lee; Svetlana Chapoval; Wei Liu; Qingsheng Chen; Anthony J Coyle; José M Lora; Dominic Picarella; Robert J Homer; Jack A Elias Journal: J Clin Invest Date: 2005-11-10 Impact factor: 14.808
Authors: Juliana Andrade Cardoso; André Avelino Dos Santos Junior; Maria Lucia Tiellet Nunes; Maria Antonia Zancanaro de Figueiredo; Karen Cherubini; Fernanda Gonçalves Salum Journal: Int J Dent Date: 2017-03-19
Authors: Xiaosong Liu; Xiaobing Guan; Ruiyang Chen; Hong Hua; Yang Liu; Zhimin Yan Journal: Evid Based Complement Alternat Med Date: 2012-12-04 Impact factor: 2.629