OBJECTIVE: Constitutive activation of various hormone and growth factor receptors is newly recognised as a common cause of tumour development. This study investigated the presence of any mutation or polymorphism of prolactin receptor (PRLR) in 38 patients with breast cancer. RESEARCH METHODS: Genomic DNA was extracted and PCR amplification was carried out for exon 1-10 of PRLR from tumoral and adjacent non-cancerous breast tissue of tumour specimens from 38 breast cancer patients. PCR products were analysed by SSCP and automatic sequencing for mutations. RESULTS: For the first time, A150C (Leu-->Ile) transversion at exon 6 of PRLR in tumour tissues, in adjacent non-cancerous breast tissues, and in blood samples of two (5.3%) out of 38 patients with breast cancer were detected. In contrast to this finding, no polymorphism of PRLR in blood samples of 100 normal individuals were found. CONCLUSION: Polymorphism of prolactin receptors might play a role in mammary carcinogenesis as a consequence of intracellular changes of PRLR signalling.
OBJECTIVE: Constitutive activation of various hormone and growth factor receptors is newly recognised as a common cause of tumour development. This study investigated the presence of any mutation or polymorphism of prolactin receptor (PRLR) in 38 patients with breast cancer. RESEARCH METHODS: Genomic DNA was extracted and PCR amplification was carried out for exon 1-10 of PRLR from tumoral and adjacent non-cancerous breast tissue of tumour specimens from 38 breast cancerpatients. PCR products were analysed by SSCP and automatic sequencing for mutations. RESULTS: For the first time, A150C (Leu-->Ile) transversion at exon 6 of PRLR in tumour tissues, in adjacent non-cancerous breast tissues, and in blood samples of two (5.3%) out of 38 patients with breast cancer were detected. In contrast to this finding, no polymorphism of PRLR in blood samples of 100 normal individuals were found. CONCLUSION: Polymorphism of prolactin receptors might play a role in mammary carcinogenesis as a consequence of intracellular changes of PRLR signalling.
Authors: Eric M Jacobson; Eric R Hugo; Traci R Tuttle; Ruben Papoian; Nira Ben-Jonathan Journal: Trends Endocrinol Metab Date: 2010-09-16 Impact factor: 12.015
Authors: Alyson A Fiorillo; Terry R Medler; Yvonne B Feeney; Suzanne M Wetz; Kalie L Tommerdahl; Charles V Clevenger Journal: Am J Pathol Date: 2012-11-14 Impact factor: 4.307
Authors: Alexandr Plotnikov; Bentley Varghese; Thai H Tran; Chengbao Liu; Hallgeir Rui; Serge Y Fuchs Journal: Cancer Res Date: 2009-03-10 Impact factor: 12.701
Authors: Roman L Bogorad; Carine Courtillot; Chidi Mestayer; Sophie Bernichtein; Lilya Harutyunyan; Jean-Baptiste Jomain; Anne Bachelot; Frédérique Kuttenn; Paul A Kelly; Vincent Goffin; Philippe Touraine Journal: Proc Natl Acad Sci U S A Date: 2008-09-08 Impact factor: 11.205
Authors: Lisa M Arendt; Debra E Rugowski; Tara A Grafwallner-Huseth; Maria Jose Garcia-Barchino; Hallgeir Rui; Linda A Schuler Journal: Breast Cancer Res Date: 2011-01-28 Impact factor: 6.466