Literature DB >> 15115078

Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli.

Mun-Yik Fong1, Yee-Ling Lau, Ithoi Init, I Jamaiah, A Khairul Anuar, N Rahmah.   

Abstract

The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES-120 antigen produced by the bacterium was investigated. A total of 45 human serum samples from patients infected with differenthelminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. The results from the assays revealed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.

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Year:  2003        PMID： 15115078

Source DB:  PubMed          Journal:  Southeast Asian J Trop Med Public Health        ISSN： 0125-1562            Impact factor:   0.267


  10 in total

1.  Development and evaluation of a sensitive and specific assay for diagnosis of human toxocariasis by use of three recombinant antigens (TES-26, TES-30USM, and TES-120).

Authors:  Suharni Mohamad; Norhaida Che Azmi; Rahmah Noordin
Journal:  J Clin Microbiol       Date:  2009-04-15       Impact factor: 5.948

2.  An alternative method for producing Toxocara canis second stage larvae from a paratenic host (pigeon) for mRNA extraction purpose.

Authors:  Amir Rahbar; Alireza Alborzi; Masoudreza Seifi Abad Shapoori
Journal:  J Parasit Dis       Date:  2013-06-07

3.  Production of Toxocara cati TES-120 Recombinant Antigen and Comparison with its T. canis Homolog for Serodiagnosis of Toxocariasis.

Authors:  Farzaneh Zahabiun; Seyed Mahmoud Sadjjadi; Muhammad Hafiznur Yunus; Anizah Rahumatullah; Mohammad Hosein Falaki Moghaddam; Syazwan Saidin; Rahmah Noordin
Journal:  Am J Trop Med Hyg       Date:  2015-06-01       Impact factor: 2.345

Review 4.  Update on eosinophilic meningoencephalitis and its clinical relevance.

Authors:  Carlos Graeff-Teixeira; Ana Cristina Arámburu da Silva; Kentaro Yoshimura
Journal:  Clin Microbiol Rev       Date:  2009-04       Impact factor: 26.132

5.  Development of a Luminex Bead Based Assay for Diagnosis of Toxocariasis Using Recombinant Antigens Tc-CTL-1 and Tc-TES-26.

Authors:  John P Anderson; Lisa N Rascoe; Keith Levert; Holly M Chastain; Matthew S Reed; Hilda N Rivera; Isabel McAuliffe; Bin Zhan; Ryan E Wiegand; Peter J Hotez; Patricia P Wilkins; Jan Pohl; Sukwan Handali
Journal:  PLoS Negl Trop Dis       Date:  2015-10-20

Review 6.  Helminth Infections and Cardiovascular Diseases: Toxocara Species is Contributing to the Disease.

Authors:  Mohammad Zibaei
Journal:  Curr Cardiol Rev       Date:  2017

7.  Sensitivity and specificity of recombinant proteins in Toxocara spp. for serodiagnosis in humans: Differences in adult and child populations.

Authors:  Lucas Moreira Dos Santos; Carolina Georg Magalhães; Paula de Lima Telmo; Michele Pepe Cerqueira; Rafael Amaral Donassolo; Fábio Pereira Leivas Leite; Guita Rubinsky Elefant; Luciana Farias da Costa Avila; Carlos James Scaini; Ângela Nunes Moreira; Fabricio Rochedo Conceição
Journal:  PLoS One       Date:  2018-12-13       Impact factor: 3.240

8.  The serodiagnostic potential of recombinant proteins TES-30 and TES-120 in an indirect ELISA in the diagnosis of toxocariasis in cattle, horses, and sheep.

Authors:  Lucas Moreira Dos Santos; Rafael Amaral Donassolo; Maria Elisabeth Berne; Fábio Pereira Leivas Leite; Luciana Farias da Costa Avila; Carlos James Scaini; Ângela Nunes Moreira; Fabricio Rochedo Conceição
Journal:  PLoS One       Date:  2019-03-14       Impact factor: 3.240

9.  The non-glycosylated protein of Toxocara canis MUC-1 interacts with proteins of murine macrophages.

Authors:  Rongqiong Zhou; Hongguo Jia; Zhendong Du; Aiyun Jiang; Zhenhui Song; Tao Wang; Aifang Du; Robin B Gasser; Guangxu Ma
Journal:  PLoS Negl Trop Dis       Date:  2022-09-02

10.  A new ELISA and western blot technique based on recombinant TES antigen and/or larval antigen for the detection of toxocariasis in humans.

Authors:  Marie-Kristin Raulf; Daniela Jordan; Herbert Auer; Jens M Warnecke; Bernd Lepenies; Christina Strube
Journal:  Parasitology       Date:  2020-10-23       Impact factor: 3.234
  10 in total

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