| Literature DB >> 15113563 |
Alison L Van Eenennaam1, Guofu Li, Mylavarapu Venkatramesh, Charlene Levering, Xiaosong Gong, Andrew C Jamieson, Edward J Rebar, Christine K Shewmaker, Casey C Case.
Abstract
Synthetic zinc finger transcription factors (ZFP-TFs) were designed to upregulate the expression of the endogenous Arabidopsis gamma-tocopherol methyltransferase (GMT) gene. This gene encodes the enzyme responsible for the conversion of gamma-tocopherol to alpha-tocopherol, the tocopherol species with the highest vitamin E activity. Five three-finger zinc finger protein (ZFP) DNA binding domains were constructed and proven to bind tightly to 9 bp DNA sequences located in either the promoter or coding region of the GMT gene. When these ZFPs were fused to a nuclear localization signal and the maize C1 activation domain, four of the five resulting ZFP-TFs were able to upregulate the expression of the GMT gene in leaf protoplast transient assays. Seed-specific expression of these ZFP-TFs in transgenic Arabidopsis produced several lines with a heritable elevation in seed alpha-tocopherol. These results demonstrate that engineered ZFP-TFs comprised of plant-derived elements are capable of modulating the expression of endogenous genes in plants.Entities:
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Year: 2004 PMID： 15113563 DOI： 10.1016/j.ymben.2003.11.001
Source DB: PubMed Journal: Metab Eng ISSN： 1096-7176 Impact factor: 9.783