| Literature DB >> 15113560 |
Joan Q Zhong1, Yelena Freyzon, Daniel J Ehrlich, Paul Matsudaira.
Abstract
We engineered green fluorescent protein (GFP) into affinity fluorescent proteins (aFPs) biosensors. The aFPs detect protein-protein interactions by enhanced fluorescence intensity. In a proof of principle demonstration, aFPs containing haemagglutinin (HA) tag bind specifically to the anti-HA antibody. The sensitivity and specificity is enhanced 28-fold by incorporation of aFPs into solid-phase surface.Mesh：
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Year: 2004 PMID： 15113560 DOI： 10.1016/j.bioeng.2003.10.004
Source DB: PubMed Journal: Biomol Eng ISSN： 1389-0344