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Literature DB >> 1511141

Characterization and expression of U1snRNA genes from potato.

P Vaux¹, F Guerineau, R Waugh, J W Brown.

Abstract

U1 small nuclear RNAs (U1snRNAs) occur in the nucleus of plants and animals where, complexed with several proteins in the form of U1 small nuclear ribonucleoprotein particles (U1snRNPs), they play an important role in precursor messenger RNA (pre-mRNA) splicing. Ten potato U1snRNA genes have been isolated on two genomic clones illustrating the clustering of this multigene family on the potato genome. Based on both the sequence of their coding regions and upstream regulatory elements, seven of the genes are potentially functional. The other three genes were pseudogenes with defective promoter or coding region sequences. Analysis of expression of individual cloned U1snRNA genes in transfected tobacco protoplasts was impossible due to the similarity of U1snRNA sequences in tobacco. However, by marking the coding regions with oligonucleotides or constructing chimaeric genes consisting of a potato U1snRNA promoter region and maize U5snRNA coding region, three of the U1 promoter regions were shown to be transcriptionally active.

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Year: 1992 PMID： 1511141 DOI： 10.1007/bf00040528

Source DB: PubMed Journal: Plant Mol Biol ISSN： 0167-4412 Impact factor: 4.076

55 in total

1. cDNA cloning of U1, U2, U4 and U5 snRNA families expressed in pea nuclei.

Authors: B A Hanley; M A Schuler
Journal: Nucleic Acids Res Date: 1991-04-25 Impact factor: 16.971

Review 2. A binding consensus: RNA-protein interactions in splicing, snRNPs, and sex.

Authors: I W Mattaj
Journal: Cell Date: 1989-04-07 Impact factor: 41.582

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Authors: D T Stolow; S M Berget
Journal: Proc Natl Acad Sci U S A Date: 1991-01-15 Impact factor: 11.205

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Authors: C W Smith; J G Patton; B Nadal-Ginard
Journal: Annu Rev Genet Date: 1989 Impact factor: 16.830

5. Genetic analysis of the role of human U1 snRNA in mRNA splicing: I. Effect of mutations in the highly conserved stem-loop I of U1.

Authors: C Y Yuo; A M Weiner
Journal: Genes Dev Date: 1989-05 Impact factor: 11.361

6. Characterisation of human and murine snRNP proteins by two-dimensional gel electrophoresis and phosphopeptide analysis of U1-specific 70K protein variants.

Authors: A Woppmann; T Patschinsky; P Bringmann; F Godt; R Lührmann
Journal: Nucleic Acids Res Date: 1990-08-11 Impact factor: 16.971

7. Structure and expression of the U5 snRNA gene of Arabidopsis thaliana. Conserved upstream sequence elements in plant U-RNA genes.

Authors: P Vankan; D Edoh; W Filipowicz
Journal: Nucleic Acids Res Date: 1988-11-25 Impact factor: 16.971

8. Functional expression of cloned human splicing factor SF2: homology to RNA-binding proteins, U1 70K, and Drosophila splicing regulators.

Authors: A R Krainer; A Mayeda; D Kozak; G Binns
Journal: Cell Date: 1991-07-26 Impact factor: 41.582

3. Structure and expression of a plant U1 snRNP 70K gene: alternative splicing of U1 snRNP 70K pre-mRNAs produces two different transcripts.

Authors: M Golovkin; A S Reddy
Journal: Plant Cell Date: 1996-08 Impact factor: 11.277

4. The U6 small nuclear RNA gene family of potato.

Authors: F Guerineau; R Waugh
Journal: Plant Mol Biol Date: 1993-08 Impact factor: 4.076

5. Differential expression of U5snRNA gene variants in maize (Zea mays) protoplasts.

Authors: D Leader; S Connelly; W Filipowicz; R Waugh; J W Brown
Journal: Plant Mol Biol Date: 1993-01 Impact factor: 4.076

5 in total