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Literature DB >> 15109607

4Pi-microscopy of the Golgi apparatus in live mammalian cells.

Alexander Egner¹, Sophie Verrier, Alexander Goroshkov, Hans-Dieter Söling, Stefan W Hell.

Abstract

We report the applicability of 4Pi-microscopy to live mammalian cells. Controlled interference of the counterpropagating wavefronts is possible despite the slight variations in cellular refractive index. Superresolved 3D-fluorescence imaging is exemplified with the first representation of the Golgi apparatus in a live cell at approximately 100 nm resolution.

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Year: 2004 PMID： 15109607 DOI： 10.1016/j.jsb.2003.10.006

Source DB: PubMed Journal: J Struct Biol ISSN： 1047-8477 Impact factor: 2.867

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Cited

17 in total

1. Cooperative 4Pi excitation and detection yields sevenfold sharper optical sections in live-cell microscopy.

Authors: Hilmar Gugel; Jörg Bewersdorf; Stefan Jakobs; Johann Engelhardt; Rafael Storz; Stefan W Hell
Journal: Biophys J Date: 2004-09-17 Impact factor: 4.033

2. H2AX chromatin structures and their response to DNA damage revealed by 4Pi microscopy.

Authors: Jörg Bewersdorf; Brian T Bennett; Kendall L Knight
Journal: Proc Natl Acad Sci U S A Date: 2006-11-16 Impact factor: 11.205

Review 3. Organization of interphase chromatin.

Authors: Rachel A Horowitz-Scherer; Christopher L Woodcock
Journal: Chromosoma Date: 2005-12-17 Impact factor: 4.316

4. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy.

Authors: Samuel T Hess; Thanu P K Girirajan; Michael D Mason
Journal: Biophys J Date: 2006-09-15 Impact factor: 4.033

5. Differential evanescence nanometry: live-cell fluorescence measurements with 10-nm axial resolution on the plasma membrane.

Authors: Saveez Saffarian; Tomas Kirchhausen
Journal: Biophys J Date: 2007-11-09 Impact factor: 4.033

6. Expedited approaches to whole cell electron tomography and organelle mark-up in situ in high-pressure frozen pancreatic islets.

Authors: Andrew B Noske; Adam J Costin; Garry P Morgan; Brad J Marsh
Journal: J Struct Biol Date: 2007-09-29 Impact factor: 2.867

4Pi-microscopy of the Golgi apparatus in live mammalian cells.

1. Cooperative 4Pi excitation and detection yields sevenfold sharper optical sections in live-cell microscopy.

2. H2AX chromatin structures and their response to DNA damage revealed by 4Pi microscopy.

Review 3. Organization of interphase chromatin.

4. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy.

5. Differential evanescence nanometry: live-cell fluorescence measurements with 10-nm axial resolution on the plasma membrane.

6. Expedited approaches to whole cell electron tomography and organelle mark-up in situ in high-pressure frozen pancreatic islets.

7. I5S: wide-field light microscopy with 100-nm-scale resolution in three dimensions.

8. Two-color nanoscopy of three-dimensional volumes by 4Pi detection of stochastically switched fluorophores.

Review 9. Optical imaging of nanoscale cellular structures.

Review 10. A guide to super-resolution fluorescence microscopy.