Literature DB >> 1510960

Secondary structure of human interleukin 2 from 3D heteronuclear NMR experiments.

H R Mott1, P C Driscoll, J Boyd, R M Cooke, M P Weir, I D Campbell.   

Abstract

Recombinant 15N-labeled human interleukin 2 (IL-2) has been studied by 2D and 3D NMR using uniformly 15N-labeled protein. Assignment of the backbone resonances has enabled the secondary structure of the protein to be defined. The secondary structure was found to consist of four alpha-helical regions and a short section of antiparallel beta-sheet. This structure is more similar to recent published structures of interleukin 4 and granulocyte-macrophage colony-stimulating factor than to a structure of IL-2 previously obtained from low-resolution X-ray diffraction data.

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Year:  1992        PMID： 1510960     DOI： 10.1021/bi00148a040

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  6 in total

1.  1H, 15N, and 13C resonance assignments of human interleukin-2.

Authors:  David C Fry; S Donald Emerson; Chao-Min Liu; Robert Palermo
Journal:  J Biomol NMR       Date:  2005-07       Impact factor: 2.835

2.  Nitric oxide and thiol redox regulation of Janus kinase activity.

Authors:  R J Duhé; G A Evans; R A Erwin; R A Kirken; G W Cox; W L Farrar
Journal:  Proc Natl Acad Sci U S A       Date:  1998-01-06       Impact factor: 11.205

3.  NMR characterization of interleukin-2 in complexes with the IL-2Ralpha receptor component, and with low molecular weight compounds that inhibit the IL-2/IL-Ralpha interaction.

Authors:  S Donald Emerson; Robert Palermo; Chao-Min Liu; Jefferson W Tilley; Li Chen; Waleed Danho; Vincent S Madison; David N Greeley; Grace Ju; David C Fry
Journal:  Protein Sci       Date:  2003-04       Impact factor: 6.725

4.  Hematopoietic cytokines: similarities and differences in the structures, with implications for receptor binding.

Authors:  A Wlodawer; A Pavlovsky; A Gustchina
Journal:  Protein Sci       Date:  1993-09       Impact factor: 6.725

5.  The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data.

Authors:  D S Wishart; B D Sykes
Journal:  J Biomol NMR       Date:  1994-03       Impact factor: 2.835

6.  Two distinct functional sites of human interleukin 4 are identified by variants impaired in either receptor binding or receptor activation.

Authors:  N Kruse; B J Shen; S Arnold; H P Tony; T Müller; W Sebald
Journal:  EMBO J       Date:  1993-12-15       Impact factor: 11.598
  6 in total

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