M E M Thompson-Cree1, M R Stevenson, M D Shields, M Ennis. 1. Department of Clinical Biochemistry, Institute of Clinical Science, The Queen's University of Belfast, Grosvenor Road, Belfast BT12 6BJ, Northern Ireland, UK. l.cree@qub.ac.uk
Abstract
BACKGROUND: Changes in the immune and inflammatory response are induced by smoking tobacco but underlying mechanisms remain to be elucidated. OBJECTIVE: This study investigated the effect of nicotine agonists on histamine release from human basophils. METHODS: Peripheral blood basophils were obtained from healthy volunteers. The effect of the nicotine agonists [-]-1-methyl-2-[3-pyridyl]pyrrolidine and (+)-nicotine di-p-toluoyltartrate salt on cell viability and anti-IgE induced histamine release was investigated. RESULTS: Cell viability was not altered by preincubation with the agents for 15 min. Anti-IgE induced histamine release was significantly inhibited by preincubation (15 min, 37 degrees C) with [-]-1-methyl-2-[3-pyridyl]pyrrolidine at the highest concentration tested 10(-)3 M (p<0.01). Preincubation (15 min, 37 degrees C) with (+)-nicotine di-p-toluoyltartrate salt significantly inhibited anti-IgE induced histamine release at 10(-3)M and 10(-5) M (p<0.05). CONCLUSIONS: This study has demonstrated that nicotine agonists inhibit histamine release from human basophils. Further studies examining the effect of smoking on basophil activation are required.
BACKGROUND: Changes in the immune and inflammatory response are induced by smoking tobacco but underlying mechanisms remain to be elucidated. OBJECTIVE: This study investigated the effect of nicotine agonists on histamine release from human basophils. METHODS: Peripheral blood basophils were obtained from healthy volunteers. The effect of the nicotine agonists [-]-1-methyl-2-[3-pyridyl]pyrrolidine and (+)-nicotine di-p-toluoyltartrate salt on cell viability and anti-IgE induced histamine release was investigated. RESULTS: Cell viability was not altered by preincubation with the agents for 15 min. Anti-IgE induced histamine release was significantly inhibited by preincubation (15 min, 37 degrees C) with [-]-1-methyl-2-[3-pyridyl]pyrrolidine at the highest concentration tested 10(-)3 M (p<0.01). Preincubation (15 min, 37 degrees C) with (+)-nicotine di-p-toluoyltartrate salt significantly inhibited anti-IgE induced histamine release at 10(-3)M and 10(-5) M (p<0.05). CONCLUSIONS: This study has demonstrated that nicotine agonists inhibit histamine release from human basophils. Further studies examining the effect of smoking on basophil activation are required.
Authors: Michael B Steinberg; Ayse Akincigil; Eun Jung Kim; Rory Shallis; Cristine D Delnevo Journal: Am J Prev Med Date: 2015-12-15 Impact factor: 5.043