| Literature DB >> 15103082 |
Tetsuro Hirose1, Tetsuya Miyamoto, Junichi Obokata, Masahiro Sugiura.
Abstract
RNA editing in higher plant chloroplasts involves C-to-U conversion at ~30 specific sites. In vitro systems supporting accurate editing have been developed from tobacco and pea chloroplasts. mRNA substrates labeled with 32P at C residues to be edited provide sensitive detection of editing activity in vitro. The present systems allow the rapid identification of cis-elements using mutated mRNA substrates and trans-acting factors by ultraviolet crosslinking.Entities:
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Year: 2004 PMID： 15103082 DOI： 10.1385/1-59259-775-0:333
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745