Craig J Slawecki1, Jennifer Roth. 1. Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California 92037, USA. cslawecki@scripps.edu
Abstract
BACKGROUND: Early life alcohol use is associated with increased alcoholism risk. It has been suggested that alterations in the sensitivity of adolescents to the acute effects of ethanol may contribute to this risk by promoting excessive intake. However, an enhanced propensity for developing ethanol dependence or withdrawal-related behavior could also contribute to increased risk. The objective of these studies was to compare the appearance of ethanol withdrawal-related behaviors in adolescent and adult rats. METHODS: Male Sprague-Dawley rats were exposed to ethanol vapor (12 hr/day) for 12 or 14 days during adolescence or adulthood. In the first study, locomotor activity was assessed after 2, 4, 7, 10, and 14 days of ethanol exposure. In the second study, open field behavior was assessed after 5 or 12 days of ethanol exposure. In follow-up studies, changes in sucrose preference during ethanol withdrawal and motor activity during food restriction were assessed in adolescent rats. Withdrawal assessments were made 7 to 9 hr after daily exposure ended. RESULTS: Hypoactivity emerged rapidly in adolescent rats during ethanol withdrawal in activity tests, but comparable reductions were not found in adult rats. However, hypoactivity developed in both adolescents and adults in the novel open field. Enhanced anxiety-like behavior in the open field was not observed in either age group during withdrawal. Finally, sucrose preference was unchanged during ethanol withdrawal, and food restriction increased motor activity in adolescent rats. CONCLUSIONS: These data confirm that symptoms of withdrawal may be differentially expressed in adolescent and adult rats. However, discrepancies in hypoactivity between studies suggest that assessment in a novel versus familiar environment may influence the expression of withdrawal-related behaviors.
BACKGROUND: Early life alcohol use is associated with increased alcoholism risk. It has been suggested that alterations in the sensitivity of adolescents to the acute effects of ethanol may contribute to this risk by promoting excessive intake. However, an enhanced propensity for developing ethanol dependence or withdrawal-related behavior could also contribute to increased risk. The objective of these studies was to compare the appearance of ethanol withdrawal-related behaviors in adolescent and adult rats. METHODS: Male Sprague-Dawley rats were exposed to ethanol vapor (12 hr/day) for 12 or 14 days during adolescence or adulthood. In the first study, locomotor activity was assessed after 2, 4, 7, 10, and 14 days of ethanol exposure. In the second study, open field behavior was assessed after 5 or 12 days of ethanol exposure. In follow-up studies, changes in sucrose preference during ethanol withdrawal and motor activity during food restriction were assessed in adolescent rats. Withdrawal assessments were made 7 to 9 hr after daily exposure ended. RESULTS: Hypoactivity emerged rapidly in adolescent rats during ethanol withdrawal in activity tests, but comparable reductions were not found in adult rats. However, hypoactivity developed in both adolescents and adults in the novel open field. Enhanced anxiety-like behavior in the open field was not observed in either age group during withdrawal. Finally, sucrose preference was unchanged during ethanol withdrawal, and food restriction increased motor activity in adolescent rats. CONCLUSIONS: These data confirm that symptoms of withdrawal may be differentially expressed in adolescent and adult rats. However, discrepancies in hypoactivity between studies suggest that assessment in a novel versus familiar environment may influence the expression of withdrawal-related behaviors.