| Literature DB >> 15094455 |
Marta Sidoryk1, Ewa Matyja, Anna Dybel, Magdalena Zielinska, Jacek Bogucki, Dariusz J Jaskólski, Pawel P Liberski, Pawel Kowalczyk, Jan Albrecht.
Abstract
Glutamine (Gln) is a growth determinant in neoplastic tissues. We analysed by RT-PCR the expression of mRNAs coding for the human variants of Gln transporters: ASCT2 (system ASC), SNAT1 [ATA1] (system A), SNAT3 [SN1] and SNAT5 [SN2] (system N), in samples of human malignant gliomas WHO grades III/IV (anaplastic astrocytoma and glioblastoma), glioma-derived cell cultures, brain metastases from peripheral organs, and control brain tissue. SNAT3 mRNA showed a 3-5 times stronger expression in gliomas than in metastases or control tissue, and was virtually absent from glioma cultures. Native glioblastoma immunostained positively with anti-SNAT3 antibody. The expression of ASCT2 mRNA, but not SNAT5 or SNAT1 mRNAs, was increased in all neoplastic tissues studied. Hence, increased expression of SNAT3 is a marker of primary malignant gliomas in situ.Entities:
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Year: 2004 PMID： 15094455 DOI： 10.1097/00001756-200403220-00001
Source DB: PubMed Journal: Neuroreport ISSN： 0959-4965 Impact factor: 1.837