| Literature DB >> 15094311 |
Jianhua Zhao1, Haobin Chen, Todd Davidson, Thomas Kluz, Qunwei Zhang, Max Costa.
Abstract
Using the mouse Affymetrix gene chip, we found that 1,4-alpha-glucan branching enzyme 1 (GBE1) was one of the most up-regulated genes following nickel exposure. This result was confirmed by Northern blot in two mouse cell lines, four mouse tissues, and three human cell lines. We further found that this gene was also up-regulated by cobalt, hypoxia, the iron chelator (deferoxamine, or DFO), and the prolyl hydroxylase (PH) inhibitor (dimethyloxalyglycine, DMOG), suggesting that hypoxia inducible factor-1alpha (HIF-1alpha) was involved in the up-regulation of this gene. Experiments using HIF-1alpha +/+ and HIF-1alpha -/- mouse cells demonstrated this gene was up-regulated through a HIF-1alpha-dependent hypoxic signaling pathway. Because the hypoxic signaling pathway is believed to be important in the initiation and progression of carcinogenesis, it is important to study genes regulated by this pathway.Entities:
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Year: 2004 PMID： 15094311 DOI： 10.1016/j.taap.2004.01.012
Source DB: PubMed Journal: Toxicol Appl Pharmacol ISSN： 0041-008X Impact factor: 4.219