BACKGROUND/AIMS: In this study we established an orthotopic tumour model for hepatocellular carcinoma and evaluated a non-invasive high resolution ultrasound technique for diagnosis and follow-up of intrahepatic HCC. METHODS: Orthotopic liver tumours were induced by intrahepatic tumour cell injection of 10(5) Hepa129 hepatoma cells. Tumour establishment and growth were assessed by explorative laparotomy, ultrasound technique and hepatectomy one and two weeks after tumour cell implantation. Tumour establishment was confirmed histologically in liver sections. RESULTS: Our results show that the Hepa129 hepatoma cell line is suitable for orthotopic tumour establishment and that tumours can be diagnosed correctly by ultrasound imaging in all cases as confirmed by explorative laparotomy, hepatectomy and cross-sections. Tumour diameters obtained by explorative laparotomy correlated significantly with diameters assessed by ultrasound (r=0.7; P<0.0001). Tumour burden was slightly overestimated (1.2-fold) by ultrasound one week after tumour induction and relative tumour extensions increased 1.7-fold and 1.8-fold within one week as determined by subsequent explorative laparotomy or ultrasound imaging, respectively. CONCLUSIONS: These data demonstrate in a systematic study that ultrasound imaging can be used as a reliable tool to detect and to follow up orthotopic liver tumours in this tumour model in mice.
BACKGROUND/AIMS: In this study we established an orthotopic tumour model for hepatocellular carcinoma and evaluated a non-invasive high resolution ultrasound technique for diagnosis and follow-up of intrahepatic HCC. METHODS: Orthotopic liver tumours were induced by intrahepatic tumour cell injection of 10(5) Hepa129 hepatoma cells. Tumour establishment and growth were assessed by explorative laparotomy, ultrasound technique and hepatectomy one and two weeks after tumour cell implantation. Tumour establishment was confirmed histologically in liver sections. RESULTS: Our results show that the Hepa129 hepatoma cell line is suitable for orthotopic tumour establishment and that tumours can be diagnosed correctly by ultrasound imaging in all cases as confirmed by explorative laparotomy, hepatectomy and cross-sections. Tumour diameters obtained by explorative laparotomy correlated significantly with diameters assessed by ultrasound (r=0.7; P<0.0001). Tumour burden was slightly overestimated (1.2-fold) by ultrasound one week after tumour induction and relative tumour extensions increased 1.7-fold and 1.8-fold within one week as determined by subsequent explorative laparotomy or ultrasound imaging, respectively. CONCLUSIONS: These data demonstrate in a systematic study that ultrasound imaging can be used as a reliable tool to detect and to follow up orthotopic liver tumours in this tumour model in mice.
Authors: Itziar Fernández-Domínguez; J Javier Echevarria-Uraga; Nieves Gómez; Zigmund Luka; Conrad Wagner; Shelly C Lu; José M Mato; Maria L Martínez-Chantar; Juan Rodríguez-Cuesta Journal: Ultrasound Med Biol Date: 2011-06-08 Impact factor: 2.998
Authors: Peter Dietrich; Laura Wormser; Valerie Fritz; Tatjana Seitz; Monica De Maria; Alexandra Schambony; Andreas E Kremer; Claudia Günther; Timo Itzel; Wolfgang E Thasler; Andreas Teufel; Jonel Trebicka; Arndt Hartmann; Markus F Neurath; Stephan von Hörsten; Anja K Bosserhoff; Claus Hellerbrand Journal: J Clin Invest Date: 2020-05-01 Impact factor: 14.808
Authors: Seagal Teitz-Tennenbaum; Qiao Li; Mary A Davis; Kari Wilder-Romans; Janet Hoff; Mu Li; Alfred E Chang Journal: J Immunother Date: 2009 Jul-Aug Impact factor: 4.456
Authors: Verena Ellerkamp; Sorin Armeanu-Ebinger; Julia Wenz; Steven W Warmann; Juergen Schäfer; Peter Ruck; Joerg Fuchs Journal: PLoS One Date: 2011-08-10 Impact factor: 3.240