Literature DB >> 1508713

The promoter of the tgt/sec operon in Escherichia coli is preceded by an upstream activation sequence that contains a high affinity FIS binding site.

R K Slany1, H Kersten.   

Abstract

The tgt/sec operon in E. coli consists of five genes: queA, tgt, ORF12, secD, and secF. QueA and Tgt participate in the biosynthesis of the hypermodified t-RNA nucleoside Queuosine, whereas SecD and SecF are involved in protein secretion. Examination of the promoter region of the operon showed structural similarity to promoter regions of the rrn-operons. An upstream activation sequence (UAS) containing a potential binding site for the factor of inversion stimulation (FIS) was found. Gel retardation assays and DNaseI footprinting indicated, that FIS binds specifically and with high affinity to a site centred at position -58. Binding of FIS caused bending of the DNA, as deduced from circular permutation analysis. Various 5' deletion mutants of the promoter region were constructed and fused to a lacZ reporter gene to determine the influence of the UAS element on the promoter strength. An approximately two-fold activation of the promoter by the UAS element was observed.

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Year:  1992        PMID： 1508713      PMCID： PMC334125          DOI： 10.1093/nar/20.16.4193

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  29 in total

1.  Analysis of the Fis-dependent and Fis-independent transcription activation mechanisms of the Escherichia coli ribosomal RNA P1 promoter.

Authors:  M Zacharias; H U Göringer; R Wagner
Journal:  Biochemistry       Date:  1992-03-10       Impact factor: 3.162

2.  The FIS protein binds and bends the origin of chromosomal DNA replication, oriC, of Escherichia coli.

Authors:  H Gille; J B Egan; A Roth; W Messer
Journal:  Nucleic Acids Res       Date:  1991-08-11       Impact factor: 16.971

3.  Involvement of Fis protein in replication of the Escherichia coli chromosome.

Authors:  M Filutowicz; W Ross; J Wild; R L Gourse
Journal:  J Bacteriol       Date:  1992-01       Impact factor: 3.490

4.  Three-dimensional structure of the E. coli DNA-binding protein FIS.

Authors:  D Kostrewa; J Granzin; C Koch; H W Choe; S Raghunathan; W Wolf; J Labahn; R Kahmann; W Saenger
Journal:  Nature       Date:  1991-01-10       Impact factor: 49.962

5.  Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes.

Authors:  K Reuter; R Slany; F Ullrich; H Kersten
Journal:  J Bacteriol       Date:  1991-04       Impact factor: 3.490

6.  Purification and properties of the Escherichia coli host factor required for inversion of the G segment in bacteriophage Mu.

Authors:  C Koch; R Kahmann
Journal:  J Biol Chem       Date:  1986-11-25       Impact factor: 5.157

7.  The trmA promoter has regulatory features and sequence elements in common with the rRNA P1 promoter family of Escherichia coli.

Authors:  C Gustafsson; P H Lindström; T G Hagervall; K B Esberg; G R Björk
Journal:  J Bacteriol       Date:  1991-03       Impact factor: 3.490

8.  The role of FIS in trans activation of stable RNA operons of E. coli.

Authors:  L Nilsson; A Vanet; E Vijgenboom; L Bosch
Journal:  EMBO J       Date:  1990-03       Impact factor: 11.598

9.  Identification of two functional regions in Fis: the N-terminus is required to promote Hin-mediated DNA inversion but not lambda excision.

Authors:  R Osuna; S E Finkel; R C Johnson
Journal:  EMBO J       Date:  1991-06       Impact factor: 11.598

10.  The E.coli fis promoter is subject to stringent control and autoregulation.

Authors:  O Ninnemann; C Koch; R Kahmann
Journal:  EMBO J       Date:  1992-03       Impact factor: 11.598
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  10 in total

Review 1.  Consensus sequence Zen.

Authors:  Thomas D Schneider
Journal:  Appl Bioinformatics       Date:  2002

2.  Fis activates the RpoS-dependent stationary-phase expression of proP in Escherichia coli.

Authors:  J Xu; R C Johnson
Journal:  J Bacteriol       Date:  1995-09       Impact factor: 3.490

3.  Genetic and molecular characterization of the Escherichia coli secD operon and its products.

Authors:  K J Pogliano; J Beckwith
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490

4.  Sequence walkers: a graphical method to display how binding proteins interact with DNA or RNA sequences.

Authors:  T D Schneider
Journal:  Nucleic Acids Res       Date:  1997-11-01       Impact factor: 16.971

5.  Identification of the miaB gene, involved in methylthiolation of isopentenylated A37 derivatives in the tRNA of Salmonella typhimurium and Escherichia coli.

Authors:  B Esberg; H C Leung; H C Tsui; G R Björk; M E Winkler
Journal:  J Bacteriol       Date:  1999-12       Impact factor: 3.490

6.  Non-canonical sequence elements in the promoter structure. Cluster analysis of promoters recognized by Escherichia coli RNA polymerase.

Authors:  O N Ozoline; A A Deev; M V Arkhipova
Journal:  Nucleic Acids Res       Date:  1997-12-01       Impact factor: 16.971

7.  Information analysis of Fis binding sites.

Authors:  P N Hengen; S L Bartram; L E Stewart; T D Schneider
Journal:  Nucleic Acids Res       Date:  1997-12-15       Impact factor: 16.971

8.  vacC, a virulence-associated chromosomal locus of Shigella flexneri, is homologous to tgt, a gene encoding tRNA-guanine transglycosylase (Tgt) of Escherichia coli K-12.

Authors:  J M Durand; N Okada; T Tobe; M Watarai; I Fukuda; T Suzuki; N Nakata; K Komatsu; M Yoshikawa; C Sasakawa
Journal:  J Bacteriol       Date:  1994-08       Impact factor: 3.490

9.  Sequence, regulation, and functions of fis in Salmonella typhimurium.

Authors:  R Osuna; D Lienau; K T Hughes; R C Johnson
Journal:  J Bacteriol       Date:  1995-04       Impact factor: 3.490

10.  Correlation between binding rate constants and individual information of E. coli Fis binding sites.

Authors:  Ryan K Shultzaberger; Lindsey R Roberts; Ilya G Lyakhov; Igor A Sidorov; Andrew G Stephen; Robert J Fisher; Thomas D Schneider
Journal:  Nucleic Acids Res       Date:  2007-07-07       Impact factor: 16.971
  10 in total

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