BACKGROUND: Estrogen and progesterone regulate alpha, beta, and gamma amiloride-sensitive epithelial sodium channel (ENaC) subunit mRNA levels in female rat kidney. Renal Na(+) handling differs between males and females. Further, within females Na(+) metabolism changes during the menstrual cycle and pregnancy. Electrolyte homeostasis and extracellular fluid volume are maintained primarily by regulated transport of Na(+) via the amiloride-sensitive Na(+) channel. This study examines the role of the female gender steroids in the regulation of expression of ENaC. METHODS: We measured ENaC subunit mRNA levels in rat kidney using Northern blotting. Kidneys were taken from male and females at different ages and from adult ovariectomized rats treated with 17-beta-estradiol benzoate (estrogen) and/or progesterone for 8 or 24 hours. RESULTS: The abundance of alpha, beta, and gammaENaC mRNA was significantly higher in female compared to male rat kidneys from 10 weeks of age (P= 0.001, P= 0.004, and P= 0.02, N= 10, respectively). These differences were abolished in ovariectomized rats. Treatment of ovariectomized rats with estrogen increased alphaENaC mRNA abundance in the kidney at both 8 and 24 hours (P < 0.05, N= 6; and P < 0.05, N= 7, respectively). Progesterone inhibited the effect of estrogen on alphaENaC mRNA at 8 hours but when given alone increased gammaENaC mRNA (P < 0.05, N= 3). Neither hormone, alone or in combination, had any significant effect on betaENaC mRNA levels at 8 or 24 hours. CONCLUSION: Female gonadal steroids differentially modulate expression of ENaC subunit mRNA in the rat kidney.
BACKGROUND: Estrogen and progesterone regulate alpha, beta, and gamma amiloride-sensitive epithelial sodium channel (ENaC) subunit mRNA levels in female rat kidney. Renal Na(+) handling differs between males and females. Further, within females Na(+) metabolism changes during the menstrual cycle and pregnancy. Electrolyte homeostasis and extracellular fluid volume are maintained primarily by regulated transport of Na(+) via the amiloride-sensitive Na(+) channel. This study examines the role of the female gender steroids in the regulation of expression of ENaC. METHODS: We measured ENaC subunit mRNA levels in rat kidney using Northern blotting. Kidneys were taken from male and females at different ages and from adult ovariectomized rats treated with 17-beta-estradiol benzoate (estrogen) and/or progesterone for 8 or 24 hours. RESULTS: The abundance of alpha, beta, and gammaENaC mRNA was significantly higher in female compared to male rat kidneys from 10 weeks of age (P= 0.001, P= 0.004, and P= 0.02, N= 10, respectively). These differences were abolished in ovariectomized rats. Treatment of ovariectomized rats with estrogen increased alphaENaC mRNA abundance in the kidney at both 8 and 24 hours (P < 0.05, N= 6; and P < 0.05, N= 7, respectively). Progesterone inhibited the effect of estrogen on alphaENaC mRNA at 8 hours but when given alone increased gammaENaC mRNA (P < 0.05, N= 3). Neither hormone, alone or in combination, had any significant effect on betaENaC mRNA levels at 8 or 24 hours. CONCLUSION: Female gonadal steroids differentially modulate expression of ENaC subunit mRNA in the rat kidney.
Authors: Mary Beth Brown; Karla K V Haack; Brian P Pollack; Mindy Millard-Stafford; Nael A McCarty Journal: Am J Physiol Regul Integr Comp Physiol Date: 2011-01-12 Impact factor: 3.619
Authors: Lauren G Douma; Kristen Solocinski; Meaghan R Holzworth; G Ryan Crislip; Sarah H Masten; Amber H Miller; Kit-Yan Cheng; I Jeanette Lynch; Brian D Cain; Charles S Wingo; Michelle L Gumz Journal: Am J Physiol Regul Integr Comp Physiol Date: 2018-11-14 Impact factor: 3.619
Authors: Rolando Carrisoza-Gaytan; Evan C Ray; Daniel Flores; Allison L Marciszyn; Peng Wu; Leah Liu; Arohan R Subramanya; WenHui Wang; Shaohu Sheng; Lubika J Nkashama; Jingxin Chen; Edwin K Jackson; Stephanie M Mutchler; Szilvia Heja; Donald E Kohan; Lisa M Satlin; Thomas R Kleyman Journal: JCI Insight Date: 2020-04-07