OBJECTIVES: To investigate the mechanisms of metastasis formation in human pancreatic carcinoma, we examined the angiogenic capabilities of human pancreatic cancer cell lines with different metastatic potentials and the roles of inflammatory cytokines. METHODS: Interleukin (IL)-8 secretion by human pancreatic cancer cells stimulated with IL-1alpha or IL-1 receptor antagonist (IL-1ra) was measured by enzyme-linked immunosorbent assay (ELISA). We then examined how cancer cells with different metastatic potentials influenced the proliferation and tube formation of human umbilical vein endothelial cells (HUVECs) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide dye reduction method (MTT assay) and an angiogenesis assay, respectively. We also examined the role of inflammatory cytokines in relation to tumor metastatic potential and angiogenesis. RESULTS: IL-8 secretion levels by pancreatic cancer cells were regulated by IL-1alpha and correlated with metastatic potential. Both HUVEC proliferation and tube formation were strongly enhanced by coculture with metastatic pancreatic cancer cells and were enhanced to a similar extent by culture in the presence of IL-1alpha and IL-8. In contrast, blockade of IL-1alpha or IL-8 inhibited HUVEC proliferation and angiogenesis. CONCLUSIONS: The inflammatory cytokines IL-1alpha and IL-8 may have an important role in metastasis via vascular endothelial cell proliferation and angiogenesis.
OBJECTIVES: To investigate the mechanisms of metastasis formation in humanpancreatic carcinoma, we examined the angiogenic capabilities of humanpancreatic cancer cell lines with different metastatic potentials and the roles of inflammatory cytokines. METHODS:Interleukin (IL)-8 secretion by humanpancreatic cancer cells stimulated with IL-1alpha or IL-1 receptor antagonist (IL-1ra) was measured by enzyme-linked immunosorbent assay (ELISA). We then examined how cancer cells with different metastatic potentials influenced the proliferation and tube formation of human umbilical vein endothelial cells (HUVECs) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide dye reduction method (MTT assay) and an angiogenesis assay, respectively. We also examined the role of inflammatory cytokines in relation to tumor metastatic potential and angiogenesis. RESULTS:IL-8 secretion levels by pancreatic cancer cells were regulated by IL-1alpha and correlated with metastatic potential. Both HUVEC proliferation and tube formation were strongly enhanced by coculture with metastatic pancreatic cancer cells and were enhanced to a similar extent by culture in the presence of IL-1alpha and IL-8. In contrast, blockade of IL-1alpha or IL-8 inhibited HUVEC proliferation and angiogenesis. CONCLUSIONS: The inflammatory cytokines IL-1alpha and IL-8 may have an important role in metastasis via vascular endothelial cell proliferation and angiogenesis.
Authors: Emily L Deer; Jessica González-Hernández; Jill D Coursen; Jill E Shea; Josephat Ngatia; Courtney L Scaife; Matthew A Firpo; Sean J Mulvihill Journal: Pancreas Date: 2010-05 Impact factor: 3.327
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Authors: Claudia Rubie; Vilma Oliveira Frick; Pirus Ghadjar; Mathias Wagner; Henner Grimm; Benjamin Vicinus; Christoph Justinger; Stefan Graeber; Martin K Schilling Journal: J Transl Med Date: 2010-05-10 Impact factor: 5.531
Authors: Felix Rückert; Gihan Dawelbait; Christof Winter; Arndt Hartmann; Axel Denz; Ole Ammerpohl; Michael Schroeder; Hans Konrad Schackert; Bence Sipos; Günter Klöppel; Holger Kalthoff; Hans-Detlev Saeger; Christian Pilarsky; Robert Grützmann Journal: PLoS One Date: 2010-08-19 Impact factor: 3.240
Authors: Min Li; Yuqing Zhang; Louis W Feurino; Hao Wang; William E Fisher; F Charles Brunicardi; Changyi Chen; Qizhi Yao Journal: Cancer Sci Date: 2008-02-27 Impact factor: 6.716